Institute for Intensive Research in Basic Sciences, Mahatma Gandhi University, Kottayam, Kerala, India.
Department of Chemistry, TKM College of Arts and Science, Karicode, Kerala, India.
J Sep Sci. 2017 Aug;40(16):3351-3357. doi: 10.1002/jssc.201700192. Epub 2017 Jul 17.
A simple capillary zone electrophoresis method with direct ultraviolet detection has been developed for the analysis of naturally occurring diastereomeric 2-hydroxycitric acid lactones. Using 50 mM sodium phosphate buffer of pH 7, a baseline resolution R > 3.0 was observed for all organic acids selected for the present study. This method was employed for the quantitative determination of title acids present in the plant sources namely Garcinia cambogia fruit rinds and Hibiscus sabdariffa calyx. Conversion of 2-hydroxycitric acids to their lactones on heating the above plant sources is deliberated. The Hydrolysis of hydroxycitric acid lactones in aqueous solution is reported for the first time.
建立了一种简单的毛细管区带电泳方法，可直接紫外检测，用于分析天然存在的差向 2-羟基柠檬酸内酯。使用 50mM 磷酸钠缓冲液（pH7），本研究中选择的所有有机酸均实现了基线分离 R>3.0。该方法用于定量测定植物来源中的标题酸，即藤黄果实果皮和玫瑰茄花萼。讨论了加热上述植物来源时 2-羟基柠檬酸转化为内酯的情况。首次报道了在水溶液中水解羟基柠檬酸内酯。
