Jayaprakasha G K, Sakariah K K
Human Resource Development, Central Food Technological Research Institute, Mysore 570 013, India.
J Pharm Biomed Anal. 2002 Apr 15;28(2):379-84. doi: 10.1016/s0731-7085(01)00623-9.
Organic acids in leaves and rinds of Garcinia indica (Kokam) were determined by high-performance liquid chromatography. The major organic acid in leaves and rinds has been found to be (-)-hydroxycitric acid present to the extent of 4.1-4.6 and 10.3-12.7%, respectively, by isocratic elution with 8 mM sulfuric acid as mobile phase with a flow rate of 1.0 ml/min using UV detection at 210 nm. Hydroxycitric acid lactone and citric acid are present in leaves and rinds in minor quantities. This method has been shown to be very reproducible with the coefficient of variation ranging from 2.8 to 4.2%. This is the first report on the composition of organic acids in the leaves and rinds of G. indica by HPLC.
采用高效液相色谱法测定了藤黄果（印度藤黄）叶片和果皮中的有机酸。以8 mM硫酸为流动相，流速为1.0 ml/min，在210 nm处进行紫外检测，通过等度洗脱发现叶片和果皮中的主要有机酸为（-）-羟基柠檬酸，其含量分别为4.1 - 4.6%和10.3 - 12.7%。叶片和果皮中还含有少量的羟基柠檬酸内酯和柠檬酸。该方法具有很高的重现性，变异系数在2.8%至4.2%之间。这是首次关于采用高效液相色谱法分析印度藤黄叶片和果皮中有机酸成分的报告。
