Can Merve Şahin, Baykan Hayriye, Baykan Özgür, Erensoy Nevin, Karlıdere Tunay
Hopa State Hospital, Department of Psychiatry, Artvin, Turkey,
Psychiatr Danub. 2017 Jun;29(2):179-185. doi: 10.24869/psyd.2017.179.
The aim of this study is to evaluate vitamin D levels and rs2228570 (FokI) polymorphism of vitamin D in patients with established diagnosis of major depressive disorder in order to investigate the impact of vitamin D levels and genetic polymorphisms on etiology and/or severity of the disease.
The study included 86 patients who were diagnosed with major depressive disorder in Hospital of Balıkesir University Faculty of Medicine, Department of Psychiatry, and 89 healthy volunteers with similar age, sex, education level and BMI. Psychiatric diagnosis was established by using Structured Clinical Interview for DSM-IV Axis I Disorders (SCID-I). For clinical evaluation, sociodemographic data form, Hamilton Depression Rating Scale, Hamilton Anxiety Scale were used. Blood samples were drawn after 12 hours of fasting from the patients volunteered and the control group who were given their informed consent for participation in the study. Vitamin D levels were determined by using the method of ECLIA (Electrochemiluminescent immunoassay). Genotype analysis was performed using the method of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP).
In our study, median vitamin D levels (min-max) of the patient and control groups were 10.3 ng/mL (3.0-42.1) and 11.4 ng/mL (3.0-38.8), respectively. Statistically significant differences as for vitamin D levels between groups were not detected (p=0.729). Similiarly no statistically significant difference between groups in genotype distribution was observed (p=0.396).
In conclusion, our findings do not support the relationship between depression, vitamin D levels and Fok 1 polymorphism of vitamin D receptor. To test these hypotheses in the light of literature we need further studies to be performed with large number of patients.
本研究旨在评估已确诊为重度抑郁症患者的维生素D水平及维生素D的rs2228570（FokI）基因多态性，以调查维生素D水平和基因多态性对该疾病病因和/或严重程度的影响。
本研究纳入了86例在巴勒克埃西尔大学医学院精神病科被诊断为重度抑郁症的患者，以及89名年龄、性别、教育水平和体重指数相似的健康志愿者。采用《精神疾病诊断与统计手册》第四版轴I障碍的结构化临床访谈（SCID-I）进行精神科诊断。临床评估使用社会人口学数据表、汉密尔顿抑郁量表、汉密尔顿焦虑量表。在患者和对照组自愿参与研究并签署知情同意书后，禁食12小时采集血样。采用电化学发光免疫分析法（ECLIA）测定维生素D水平。使用聚合酶链反应-限制性片段长度多态性方法（PCR-RFLP）进行基因型分析。
在我们的研究中，患者组和对照组的维生素D水平中位数（最小值-最大值）分别为10.3 ng/mL（3.0-42.1）和11.4 ng/mL（3.0-38.8）。未检测到两组间维生素D水平的统计学显著差异（p=0.729）。同样，两组间基因型分布也未观察到统计学显著差异（p=0.396）。
总之，我们的研究结果不支持抑郁症、维生素D水平与维生素D受体Fok 1基因多态性之间的关系。为根据文献验证这些假设，我们需要对大量患者进行进一步研究。
