Lobão T N, Campos G B, Selis N N, Amorim A T, Souza S G, Mafra S S, Pereira L S, Dos Santos D B, Figueiredo T B, Marques L M, Timenetsky J
Instituto de Ciências Biomédicas,Departamento de Microbiologia,Universidade de São Paulo,Brazil.
Instituto Multidisciplinar em Saúde,Núcleo de Tecnologia em Saúde,Universidade Federal da Bahia,Brazil.
Epidemiol Infect. 2017 Aug;145(11):2341-2351. doi: 10.1017/S0950268817001145. Epub 2017 Jun 22.
Ureaplasma urealyticum and U. parvum have been associated with genital infections. The purpose of this study was to detect the presence of ureaplasmas and other sexually transmitted infections in sexually active women from Brazil and relate these data to demographic and sexual health, and cytokines IL-6 and IL-1β. Samples of cervical swab of 302 women were examined at the Family Health Units in Vitória da Conquista. The frequency of detection by conventional PCR was 76·2% for Mollicutes. In qPCR, the frequency found was 16·6% for U. urealyticum and 60·6% U. parvum and the bacterial load of these microorganisms was not significantly associated with signs and symptoms of genital infection. The frequency found for Trichomonas vaginalis, Neisseria gonorrhoeae, Gardnerella vaginalis and Chlamydia trachomatis was 3·0%, 21·5%, 42·4% and 1·7%, respectively. Higher levels of IL-1β were associated with control women colonized by U. urealyticum and U. parvum. Increased levels of IL-6 were associated with women who exhibited U. parvum. Sexually active women, with more than one sexual partner in the last 3 months, living in a rural area were associated with increased odds of certain U. parvum serovar infection.
解脲脲原体和微小脲原体与生殖器感染有关。本研究的目的是检测来自巴西的性活跃女性中脲原体和其他性传播感染的存在情况，并将这些数据与人口统计学和性健康以及细胞因子白细胞介素-6（IL-6）和白细胞介素-1β（IL-1β）相关联。在维多利亚达孔基斯塔的家庭健康单位对302名女性的宫颈拭子样本进行了检查。通过常规聚合酶链反应（PCR）检测到支原体的频率为76.2%。在定量PCR（qPCR）中，解脲脲原体的检出频率为16.6%，微小脲原体为60.6%，这些微生物的细菌载量与生殖器感染的体征和症状无显著关联。阴道毛滴虫、淋病奈瑟菌、阴道加德纳菌和沙眼衣原体的检出频率分别为3.0%、21.5%、42.4%和1.7%。较高水平的IL-1β与被解脲脲原体和微小脲原体定植的对照女性有关。IL-6水平升高与检出微小脲原体的女性有关。在过去3个月内有多个性伴侣、居住在农村地区的性活跃女性感染某些微小脲原体血清型的几率增加。
