Liu Yanling, Chen Liangming, Liu Yuqiang, Dai Huimin, He Jun, Kang Haiyan, Pan Gen, Huang Jie, Qiu Zeyu, Wang Qi, Hu Jinlong, Liu Linglong, Chen Yezhi, Cheng Xianian, Jiang Ling, Wan Jianmin
National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China.
Rice (N Y). 2016 Dec;9(1):27. doi: 10.1186/s12284-016-0096-3. Epub 2016 May 31.
Brown planthopper (BPH) is the most destructive insect in rice production. Breeding of resistant cultivars is the most cost-effective and environment-friendly strategy for BPH management; however, resistant cultivars are currently hampered by the rapid breakdown of BPH resistance. Thus, there is an urgent need to use more effective BPH resistance genes or pyramiding different resistance genes to develop more durable resistant rice cultivars.
Here a dominant BPH resistance gene Bph27(t) were introgressed into a susceptible commercial japonica variety Ningjing3 (NJ3) and indica variety 93-11 using marker-assisted selection (MAS), respectively. Further, Bph27(t) and a durable BPH resistance gene Bph3 was pyramided by intercrossing single-gene introgressed lines through MAS. The introgression of BPH resistance genes significantly improved the BPH resistance and reduced the yield loss caused by BPH.
The development of single and two genes pyramided lines in this study provides innovative resources for molecular breeding of durable BPH-resistant rice cultivars and BPH management through resistant cultivars.
褐飞虱是水稻生产中最具破坏力的害虫。培育抗性品种是褐飞虱治理中最具成本效益且环境友好的策略；然而，目前抗性品种因褐飞虱抗性的快速丧失而受到阻碍。因此，迫切需要利用更有效的褐飞虱抗性基因或聚合不同的抗性基因来培育更持久抗性的水稻品种。
在此，通过标记辅助选择（MAS），分别将一个显性褐飞虱抗性基因Bph27(t)导入感病的粳稻商业品种宁粳3号（NJ3）和籼稻品种93-11中。此外，通过MAS将单基因导入系进行杂交，使Bph27(t)和一个持久的褐飞虱抗性基因Bph3聚合。褐飞虱抗性基因的导入显著提高了对褐飞虱的抗性，并减少了褐飞虱造成的产量损失。
本研究中单个和两个基因聚合系的培育为通过抗性品种进行持久抗褐飞虱水稻品种的分子育种和褐飞虱治理提供了创新资源。
