活细胞中环磷酸腺苷及其他腺苷磷酸的差异检测与定量分析。

Differential detection and quantification of cyclic AMP and other adenosine phosphates in live cells.

作者信息

Das Sujoy, Sarkar Himadri Sekhar, Uddin Md Raihan, Rissanen Kari, Mandal Sukhendu, Sahoo Prithidipa

机构信息

Department of Chemistry, Visva-Bharati university, Santiniketan, 731235, India.

出版信息

Chem Commun (Camb). 2017 Jul 4;53(54):7600-7603. doi: 10.1039/c7cc02935g.

DOI:10.1039/c7cc02935g

PMID:28639644

Abstract

A new naphthol-based rhodamine derivative (NpRD) has been developed for the selective and differential detection of adenosine 3',5'-cyclic monophosphate (cAMP) and adenosine phosphates (APs) (ATP, ADP, and AMP) from other nucleotides. The simple detection and quantification of cAMP in human blood cells and in other samples based on the 'turn on' fluorescence properties of this chemosensor through colorimetry or fluorometry makes it unique for probable application in high throughput screening.

摘要

一种基于萘酚的新型罗丹明衍生物（NpRD）已被开发出来，用于从其他核苷酸中选择性、差异性地检测3',5'-环磷酸腺苷（cAMP）和腺苷磷酸（APs）（三磷酸腺苷、二磷酸腺苷和一磷酸腺苷）。基于这种化学传感器通过比色法或荧光法的“开启”荧光特性，对人体血细胞和其他样品中的cAMP进行简单检测和定量，使其在高通量筛选中具有潜在应用的独特性。