a Department of Biological Science , BITS-Pilani, K.K Birla Goa Campus , Zuarinagar , India.
b Department of Chemistry , BITS-Pilani, K.K Birla Goa Campus , Zuarinagar , India.
Artif Cells Nanomed Biotechnol. 2018 May;46(3):637-649. doi: 10.1080/21691401.2017.1337021. Epub 2017 Jun 22.
With increasing gap in the demand and supply of vital organs for transplantation there is a pressing need to bridge the gap with substitutes. One way to make substitutes is by tissue engineering which involves combining several types of synthetic or biomaterials, cells and growth factors cross-linked together to synthesize a functional scaffold for repair or replacement of non-functional organs. Nanoparticle based composites are gaining importance in tissue engineering due to their ability to enhance cell attachment and proliferation. The current study focuses on synthesizing agarose composites embedded with chitosan-coated silver nanoparticles using glutaraldehyde as the cross-linker. The synthesis of chitosan coated silver nanoparticles within the scaffold was confirmed with UV-visible spectroscopy. Physical and chemical characterization of the synthesized nanoparticles were done by XRD, FTIR, TGA and SEM. DMA showed higher mechanical strength of the scaffolds. The scaffolds showed degradation of ∼37% within a span of four weeks. The higher physical support provided by the synthesized scaffolds was shown by in-vitro cell viability assay. Broad spectrum anti-bacterial activity and superior hemocompatibility further showed the advantage it offered for growing cells. Thus a biopolymer based nanocomposite was synthesized, with intended widespread use as scaffold for engineering of soft tissues due to its enhanced biocompatibility and greater surface area for cell growth.
随着移植所需关键器官的供需差距不断扩大，迫切需要用替代品来填补这一差距。一种制造替代品的方法是组织工程学，它涉及将几种类型的合成或生物材料、细胞和生长因子交联在一起，合成一种功能性支架，用于修复或替代功能失调的器官。基于纳米粒子的复合材料由于其增强细胞附着和增殖的能力，在组织工程学中变得越来越重要。本研究专注于使用戊二醛作为交联剂，合成琼脂糖复合材料，其中嵌入壳聚糖涂层的银纳米粒子。通过紫外可见光谱证实了支架内壳聚糖涂层银纳米粒子的合成。通过 XRD、FTIR、TGA 和 SEM 对合成纳米粒子进行了物理化学特性表征。DMA 显示出支架更高的机械强度。在四周的时间内，支架降解了约 37%。体外细胞活力测定显示，合成支架提供了更高的物理支撑。广谱抗菌活性和优异的血液相容性进一步表明，它为细胞生长提供了优势。因此，合成了一种基于生物聚合物的纳米复合材料，由于其增强的生物相容性和更大的细胞生长表面积，预计将广泛用作软组织工程的支架。
