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印度桑（桑属，桑科）查尔酮合酶基因的等位基因特异性CAPS标记开发与特性分析

Allele specific CAPS marker development and characterization of chalcone synthase gene in Indian mulberry (Morus spp., family Moraceae).

作者信息

Arora Vivek, Ghosh M K, Pal Soumili, Gangopadhyay Gaurab

机构信息

Division of Plant Biology, Bose Institute (Main Campus), Kolkata, India.

Central Sericultural Research & Training Institute, Berhampore, India.

出版信息

PLoS One. 2017 Jun 22;12(6):e0179189. doi: 10.1371/journal.pone.0179189. eCollection 2017.

DOI:10.1371/journal.pone.0179189
PMID:28640826
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5480871/
Abstract

Chalcone synthase (CHS) is an essential enzyme in the phenylpropanoid pathway that catalyzes the first step in flavonoid biosynthesis in plants under diverse environmental stress. We have used CHS as a candidate gene in mulberry and developed Single Nucleotide Polymorphism (SNP) based co-dominant Cleaved Amplified Polymorphic Sequence (CAPS) marker associated with the CHS locus. The segregation pattern of the marker was studied in an F1 population derived from a hybridization program between two mulberry genotypes showing polymorphism for the CHS locus. Differential CHS activity of the recombinants has been correlated with the segregation pattern of the marker. Homology modelling and docking studies are performed for both the identified CHS alleles and correlated with respective CHS activity. Phenotyping of Powdery Mildew infected F1 population indicated a probable association with the CAPS marker.

摘要

查尔酮合酶（CHS）是苯丙烷类途径中的一种关键酶，在多种环境胁迫下催化植物类黄酮生物合成的第一步。我们已将CHS用作桑树中的候选基因，并开发了基于单核苷酸多态性（SNP）的共显性酶切扩增多态性序列（CAPS）标记，该标记与CHS基因座相关。在由两个桑树基因型杂交产生的F1群体中研究了该标记的分离模式，这两个基因型在CHS基因座上表现出多态性。重组体的不同CHS活性已与标记的分离模式相关联。对鉴定出的两个CHS等位基因进行了同源建模和对接研究，并将其与各自的CHS活性相关联。对感染白粉病的F1群体进行表型分析表明，其可能与CAPS标记有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d75/5480871/efb25cc1782f/pone.0179189.g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d75/5480871/7fd7c42ab2ea/pone.0179189.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d75/5480871/efb25cc1782f/pone.0179189.g009.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d75/5480871/25c2add7ca41/pone.0179189.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d75/5480871/1cb651883006/pone.0179189.g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d75/5480871/efb25cc1782f/pone.0179189.g009.jpg

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