Sugi Takuma
Molecular Neuroscience Research Center, Shiga University of Medical Science, Otsu, Shiga, 520-2192, Japan.
Methods Mol Biol. 2017;1630:247-254. doi: 10.1007/978-1-4939-7128-2_20.
Caenorhabditis elegans, a 1 mm long free-living nematode, is a traditional model animal for genetic investigations of various biological processes. Characteristic features that make C. elegans a powerful model of choice for eukaryotic genetic studies include its rapid life cycle, well-annotated genome, simple morphology, and transparency. Recently, genome editing technologies have been increasingly used in C. elegans, thereby facilitating their genetic analyses. Here, I introduce a protocol frequently used in C. elegans genome editing.
秀丽隐杆线虫是一种体长1毫米的自由生活线虫，是用于各种生物学过程基因研究的传统模式动物。使秀丽隐杆线虫成为真核生物基因研究有力选择模式的特征包括其快速的生命周期、注释完善的基因组、简单的形态以及透明性。最近，基因组编辑技术在秀丽隐杆线虫中得到越来越多的应用，从而促进了它们的基因分析。在此，我介绍一种秀丽隐杆线虫基因组编辑中常用的方案。
