Molecular Neuroscience Research Center, Shiga University of Medical Science, Otsu, Shiga, Japan.
Methods Mol Biol. 2023;2637:389-396. doi: 10.1007/978-1-0716-3016-7_29.
Caenorhabditis elegans, a 1 mm long free-living nematode, is a traditional model animal for genetic investigations of various biological processes. Characteristic features that make C. elegans a powerful model of choice for eukaryotic genetic studies include its rapid life cycle, well-annotated genome, simple morphology, and transparency. Recently, genome editing technologies have been increasingly used in C. elegans, thereby facilitating their genetic analyses. Here, I introduce a protocol frequently used in C. elegans genome editing.
秀丽隐杆线虫，一种 1 毫米长的自由生活线虫，是遗传研究各种生物过程的传统模式动物。使秀丽隐杆线虫成为真核生物遗传研究的有力模型的特征包括其快速的生命周期、注释良好的基因组、简单的形态和透明性。最近，基因组编辑技术在秀丽隐杆线虫中越来越多地被使用，从而促进了它们的遗传分析。在这里，我将介绍秀丽隐杆线虫基因组编辑中常用的一种方法。
