Boudreau-Pinsonneault Camille, Cayouette Michel
Cellular Neurobiology Research Unit, Institut de Recherches Cliniques de Montréal (IRCM), Montreal, QC, Canada.
Integrated Program in Neuroscience, Department of Medicine, McGill University, Montreal, QC, Canada.
Dev Dyn. 2018 Jan;247(1):10-17. doi: 10.1002/dvdy.24535. Epub 2017 Jul 13.
Recent studies reported the transfer of fluorescent labels between grafted and host cells after transplantation of photoreceptor precursor cells in the mouse retina. While clearly impacting the interpretation of transplantation studies in the retina, the potential impact of material transfer in other experimental paradigms using cell-specific labels remains uncertain. Here, we briefly review the evidence supporting material transfer in transplantation studies and discuss whether it might influence retinal cell lineage tracing experiments in developmental and regeneration studies. We also propose ways to control for the possible confounding occurrence of label exchange in such experiments. Developmental Dynamics 247:10-17, 2018. © 2017 Wiley Periodicals, Inc.
最近的研究报道，在小鼠视网膜中移植光感受器前体细胞后，荧光标记物会在移植细胞与宿主细胞之间转移。虽然这显然影响了视网膜移植研究的解读，但在其他使用细胞特异性标记物的实验范式中，物质转移的潜在影响仍不确定。在这里，我们简要回顾支持移植研究中物质转移的证据，并讨论其是否可能影响发育和再生研究中的视网膜细胞谱系追踪实验。我们还提出了在这类实验中控制标记物交换可能产生的混淆影响的方法。《发育动力学》2018年第247卷：10 - 17页。© 2017威利期刊公司。
