Pere A, Leinonen M, Väisänen-Rhen V, Rhen M, Korhonen T K
J Gen Microbiol. 1985 Jul;131(7):1705-11. doi: 10.1099/00221287-131-7-1705.
Two monoclonal antibodies specific for type-1C fimbriae of Escherichia coli were produced. In enzyme-linked immunosorbent assay and immunoblotting the antibodies, which were of the IgG1 isotype, reacted with type-1C, but not with P or type-1 fimbriae of E. coli strain KS71. Immunoblotting and immunoprecipitation of crude fimbrial extracts from 25 strains invariably gave an apparent molecular weight of 17 000 for the type-1C fimbrillin. A total of 313 E. coli strains, isolated from patients with extraintestinal infection or from faeces of healthy children, were screened for the presence of type-1C fimbriae using both the monoclonal and polyclonal antibodies. Of these, 45 (14%) strains had type-1C fimbriae, with the highest frequency (27%) on strains isolated from patients with pyelonephritis. No faecal strain had type-1C fimbriae, and the frequency on the other diagnostic groups ranged from 11 to 15%. Thus, no direct correlation between type-1C fimbriae and bacterial virulence in human extraintestinal infections was found. Type-1C fimbriae were detected on only a few E. coli serotypes, notably on all O6:K2:H1 and O22:K13:H1 strains tested.
制备了两种针对大肠杆菌1C型菌毛的单克隆抗体。在酶联免疫吸附测定和免疫印迹中，这两种IgG1同种型的抗体与1C型菌毛发生反应，但不与大肠杆菌KS71菌株的P菌毛或1型菌毛反应。对来自25个菌株的粗菌毛提取物进行免疫印迹和免疫沉淀，结果显示1C型菌毛蛋白的表观分子量始终为17000。使用单克隆抗体和多克隆抗体对总共313株从肠外感染患者或健康儿童粪便中分离出的大肠杆菌菌株进行1C型菌毛检测。其中，45株（14%）菌株有1C型菌毛，在从肾盂肾炎患者分离出的菌株中频率最高（27%）。粪便菌株均无1C型菌毛，其他诊断组中的频率在11%至15%之间。因此，未发现1C型菌毛与人类肠外感染中细菌毒力之间存在直接关联。仅在少数大肠杆菌血清型中检测到1C型菌毛，特别是在所测试的所有O6:K2:H1和O22:K13:H1菌株中。
