Genome-Wide Analysis of Genes in L. and Positive Modulation of Osmotic Tolerance by .

Myeloblastosis (MYB)-related transcription factors comprise a large subfamily of the MYB family. They play significant roles in plant development and in stress responses. However, MYB-related proteins have not been comprehensively investigated in rapeseed ( L.). In the present study, a genome-wide analysis of MYB-related transcription factors was performed in rapeseed. We identified 251 (BnMYB)-related members, which were divided phylogenetically into five clades. Evolutionary analysis suggested that whole genome duplication and segmental duplication events have played a significant role in the expansion of gene family. Selective pressure of genes was estimated using the ratio, which indicated that genes underwent strong purifying selection during evolution. analysis showed that various development-associated, phytohormone-responsive, and stress-related -acting regulatory elements were enriched in the promoter regions of genes. Furthermore, genes with tissue or organ-specific, stress-responsive expression patterns were identified in based on temporospatial and abiotic stress expression profiles. Among the stress-responsive genes, was strongly induced by drought stress, and was therefore selected for functional study. Rapeseed seedlings overexpressing showed improved resistance to osmotic stress. Our findings not only lay a foundation for further functional characterization of genes, but also provide valuable clues to determine candidate genes for future genetic improvement of .