Department of Microbiology, Faculty of Dentistry, University of Granada, Granada, Spain.
Department of Restorative Dentistry, Faculty of Dental Surgery, University of Malta, Malta.
J Dent. 2017 Sep;64:45-51. doi: 10.1016/j.jdent.2017.06.006. Epub 2017 Jun 20.
Characterization of a number of pulp capping materials and assessment of the leachate for elemental composition, antimicrobial activity and cell proliferation and expression.
Three experimental light curable pulp-capping materials, Theracal and Biodentine were characterized by scanning electron microscopy, energy dispersive spectroscopy and X-ray diffraction. The elemental composition of the leachate formed after 24h was assessed by inductively coupled plasma (ICP). The antimicrobial activity of the leachate was determined by the minimum inhibitory concentration (MIC) against multispecies suspensions of Streptococcus mutans ATCC 25175, Streptococcus gordonii ATCC 33478 and Streptococcus sobrinus ATCC 33399. Cell proliferation and cell metabolic function over the material leachate was assessed by an indirect contact test using 3-(4,5 dimethylthiazolyl-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
The hydration behavior of the test materials varied with Biodentine being the most reactive and releasing the highest amount of calcium ions in solution. All materials tested except the unfilled resin exhibited depletion of phosphate ions from the solution indicating interaction of the materials with the media. Regardless the different material characteristics, there was a similar antimicrobial activity and cellular activity. All the materials exhibited no antimicrobial activity and were initially cytotoxic with cell metabolic function improving after 3days.
The development of light curable tricalcium silicate-based pulp capping materials is important to improve the bonding to the final resin restoration. Testing of both antimicrobial activity and biological behavior is critical for material development. The experimental light curable materials exhibited promising biological properties but require further development to enhance the antimicrobial characteristics.
对几种牙髓覆盖材料进行特性描述，并对浸提液的元素组成、抗菌活性、细胞增殖和表达进行评估。
通过扫描电子显微镜、能量色散光谱和 X 射线衍射对三种实验性光固化牙髓覆盖材料（Theracal 和 Biodentine）进行了表征。通过电感耦合等离子体（ICP）评估 24 小时后形成的浸提液的元素组成。通过最小抑菌浓度（MIC）测定浸提液对变异链球菌 ATCC 25175、戈登链球菌 ATCC 33478 和远缘链球菌 ATCC 33399 多菌种悬浮液的抗菌活性。通过 3-(4,5-二甲基噻唑基-2)-2,5-二苯基四氮唑溴盐（MTT）测定间接接触试验中材料浸提液对细胞增殖和细胞代谢功能的影响。
测试材料的水合行为各不相同，Biodentine 反应性最强，溶液中释放的钙离子最多。除未填充树脂外，所有测试材料均从溶液中耗尽磷酸盐离子，表明材料与介质相互作用。尽管材料特性不同，但抗菌活性和细胞活性相似。所有材料均无抗菌活性，最初具有细胞毒性，细胞代谢功能在 3 天后得到改善。
开发光固化硅酸三钙基牙髓覆盖材料对于提高与最终树脂修复体的粘结性很重要。测试抗菌活性和生物行为对于材料开发至关重要。实验性光固化材料表现出有前途的生物学特性，但需要进一步开发以增强抗菌特性。
