Daoud Amal, Ben Mefteh Fedia, Mnafgui Kais, Turki Mouna, Jmal Salwa, Ben Amar Rawdha, Ayadi Fatma, ElFeki Abdelfattah, Abid Leila, Rateb Mostafa E, Belbahri Lassaad, Kadri Adel, Gharsallah Neji
Laboratory of Plant Biotechnology, Faculty of Sciences of Sfax, B.P.1171, 3000 Sfax, Tunisia.
Laboratory of Plant Biotechnology, Faculty of Sciences of Sfax, B.P.1171, 3000 Sfax, Tunisia; Laboratory of Animal Physiology, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 95, Sfax 3052, Tunisia.
Exp Toxicol Pathol. 2017 Oct 2;69(8):656-665. doi: 10.1016/j.etp.2017.06.004. Epub 2017 Jun 20.
The present study aimed to examine the putative preventive effect of the ethanolic extract Date Palm Pollen (DPP, Phoenix dactylifera L., family Arecaceae) on isoproterenol-induced myocardial infarction (MI) in rats. Twenty four rats were randomly divided into four groups including control. They were treated with DPP extract (400mg/kg) and clopidogrel (0.2mg/kg) for 7days followed by myocardial injury induction using subcutaneous isoproterenol (100mg/kg) with an interval of 24h for two days (6th and 7th day). Administration of isoproterenol exhibited indicative changes in the ECG pattern evidenced by significant elevation of ST-segment and cardiac injury markers viz.; troponin-T, creatine phosphokinase (CPK), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) by 315%, 71%, 64% and 170%, respectively as compared to control. Additionally, the angiotensin-converting enzyme (ACE) activity in plasma was increased by 33% associated to histological myocardial necrosis. However, pre-co-treatment with DPP extract improved the cardiac biomarkers injury, normalized cardiac function indices and prevented the ventricular remodeling process through inhibition of ACE activity by 34% and the inhibition of the generation of radical oxygen species. Extensive characterization of this DPP extract using LC-HRMS revealed numerous flavonoids and phenols compounds which could be endowed with cardiopreventive actions. Overall, these results proved that DPP extract has preventive effects on cardiac remodeling process.
本研究旨在探讨椰枣花粉（DPP，Phoenix dactylifera L.，棕榈科）乙醇提取物对异丙肾上腺素诱导的大鼠心肌梗死（MI）的假定预防作用。24只大鼠随机分为包括对照组在内的四组。它们接受DPP提取物（400mg/kg）和氯吡格雷（0.2mg/kg）治疗7天，随后使用皮下注射异丙肾上腺素（100mg/kg）诱导心肌损伤，间隔24小时，共两天（第6天和第7天）。异丙肾上腺素给药后，心电图模式出现指示性变化，表现为ST段显著升高以及心脏损伤标志物，即肌钙蛋白-T、肌酸磷酸激酶（CPK）、丙氨酸转氨酶（ALT）和乳酸脱氢酶（LDH）分别比对照组升高315%、71%、64%和170%。此外，与组织学心肌坏死相关，血浆中的血管紧张素转换酶（ACE）活性增加了33%。然而，预先用DPP提取物治疗可改善心脏生物标志物损伤，使心脏功能指标正常化，并通过抑制34%的ACE活性和抑制活性氧的产生来防止心室重构过程。使用LC-HRMS对该DPP提取物进行的广泛表征揭示了许多可能具有心脏预防作用的黄酮类和酚类化合物。总体而言，这些结果证明DPP提取物对心脏重构过程具有预防作用。
