Ondokuz Mayis University Faculty of Dentistry, Samsun, Turkey.
Ondokuz Mayis University Faculty of Dentistry, Samsun, Turkey.
J Endod. 2017 Aug;43(8):1309-1316. doi: 10.1016/j.joen.2017.03.019. Epub 2017 Jun 22.
This study aimed to characterize the microbial communities of primary and secondary/persistent endodontic infections using high-throughput pyrosequencing from the pulverized samples.
The roots of 20 extracted human teeth with primary endodontic infection and 20 teeth with secondary/persistent endodontic infection were collected. The outer surfaces of the roots were disinfected, and whole roots were cryopulverized. 16S amplicon pyrosequencing data from the DNA extracted from the pulverized root powders were obtained, and microorganism abundance and diversity were calculated. Data were analyzed using statistical and bioinformatic methods.
Pyrosequencing analysis resulted a total of 2,606,128 sequences from 40 samples. A total of 15 phyla, 160 genera, and 368 species were detected. No significant difference between primary and secondary/persistent endodontic infections was found regarding the diversity and richness of operational taxonomic units at the phyla, genera, and species levels (P > .005).
The present study revealed that the microbial diversity of secondary/persistent endodontic infections did not differ than those of primary endodontic infections. A new archaeal species, Candidatus Nitrosoarchaeum limnia, was detected in root canals of 1 patient with primary endodontic infection for the first time.
本研究旨在通过高通量焦磷酸测序从粉碎样本中描述原发性和继发性/持续性根管感染的微生物群落。
收集 20 颗患有原发性根管感染和 20 颗患有继发性/持续性根管感染的人离体牙的牙根。根的外表面经过消毒，整个根被冷冻粉碎。从粉碎的根粉中提取 DNA 进行 16S 扩增子焦磷酸测序，计算微生物丰度和多样性。使用统计和生物信息学方法对数据进行分析。
40 个样本的焦磷酸测序分析共产生了 2606128 条序列。共检测到 15 个门、160 个属和 368 个种。在门、属和种水平上，原发性和继发性/持续性根管感染的操作分类单位的多样性和丰富度无显著差异（P>.005）。
本研究表明，继发性/持续性根管感染的微生物多样性与原发性根管感染无差异。首次在 1 例原发性根管感染患者的根管中检测到一种新的古菌种，即 Candidatus Nitrosoarchaeum limnia。
