Instituto de Salud y Ambiente del Litoral (ISAL, CONICET-UNL), Ciudad Universitaria, Paraje El Pozo s/n, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CP3000 Santa Fe, Argentina.
Instituto de Salud y Ambiente del Litoral (ISAL, CONICET-UNL), Ciudad Universitaria, Paraje El Pozo s/n, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, CP3000 Santa Fe, Argentina.
Reprod Toxicol. 2017 Oct;73:259-268. doi: 10.1016/j.reprotox.2017.06.050. Epub 2017 Jun 23.
The ovary is sensitive to disruption by the environmental estrogen Bisphenol A (BPA). Our aim was to investigate whether perinatal exposure to BPA (50μg/kgday), orally administered, affects ovarian response to exogenous gonadotrophins (PMSG or PMSG+hCG) in prepubertal female offspring. An altered response to gonadotrophins was observed in BPA-exposed rats. Increased proportion of antral follicles, altered levels of ovarian steroidogenic enzymes, gonadotropin receptors, AR and ERβ were observed in PMSG group. Besides that, in response to PMSG+hCG, a persistent high Fshr mRNA expression and a decreased number of follicles with high expression of PR before ovulation were observed. After ovulation, there was an increase in antral atretic follicles, reduced Lhcgr mRNA expression and high serum levels of E2. Therefore, an early exposure to a low dose of BPA during perinatal period induces ovarian changes leading to an altered response to exogenous gonadotropin treatment later in life.
卵巢对外源雌激素双酚 A（BPA）很敏感。我们的目的是研究围产期经口给予低剂量 BPA（50μg/kg·day）是否会影响未成年雌性后代对外源性促性腺激素（PMSG 或 PMSG+hCG）的卵巢反应。在接受 BPA 处理的大鼠中观察到对外源促性腺激素反应的改变。在 PMSG 组中，观察到窦前卵泡的比例增加，卵巢甾体生成酶、促性腺激素受体、AR 和 ERβ 的水平发生改变。此外，在对 PMSG+hCG 的反应中，在排卵前，Fshr mRNA 表达持续升高，高表达 PR 的卵泡数量减少。排卵后，窦前闭锁卵泡增加，Lhcgr mRNA 表达减少，血清 E2 水平升高。因此，围产期早期暴露于低剂量 BPA 会导致卵巢发生变化，从而导致成年后对外源促性腺激素治疗的反应发生改变。
