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一种便携式 RGB 感应小工具,使用半胱氨酸封端的 QD 作为荧光探针,实现对 Hg 的灵敏检测。

A portable RGB sensing gadget for sensitive detection of Hg using cysteamine-capped QDs as fluorescence probe.

机构信息

Chongqing Key Laboratory of Non-linear Circuit and Intelligent Information Processing, College of Electronic and Information Engineering, Southwest University, Chongqing 400715, China; Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University) Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.

School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China.

出版信息

Biosens Bioelectron. 2017 Dec 15;98:36-40. doi: 10.1016/j.bios.2017.05.032. Epub 2017 Jun 8.

Abstract

A facile one-pot method was proposed to synthesis cysteamine capped CdTe QDs with a high quantum yield 30%. In the synthesis process, nitrogen protection, ultrasonic treatment, and light irradiation were unnecessary. Additionally, potassium tellurite was used instead of Te powder as Te source, avoiding of the time-consuming Te powder dissolution process. The prepared QDs were found to be Hg sensitive. The detection linear range for Hg was from 5 to 300nM, and the detection limit was calculated to be 1nM. The detection mechanism was mainly ascribed to the electron transfer from cysteamine capped QDs to Hg, resulting in a fluorescence quenching phenomenon. In addition, we developed a portable RGB sensing gadget with the QDs as fluorescence probe for Hg detection, and the linear range was from 5 to 200nM. Furthermore, the gadget was applied for Hg detection in tap water sample. The results showed that the average recoveries in real tap water sample were in the range from 97.2% to 115.3%, which indicated that accuracy and precision were satisfactory for practical application requirements.

摘要

一种简便的一锅法被提出用于合成半胱胺封端的 CdTe QDs,其量子产率高达 30%。在合成过程中,不需要氮气保护、超声处理和光照。此外,使用亚碲酸钾代替碲粉作为碲源,避免了耗时的碲粉溶解过程。所制备的 QDs 对 Hg 具有敏感性。Hg 的检测线性范围为 5 至 300nM,检测限计算为 1nM。检测机制主要归因于半胱胺封端的 QDs 与 Hg 之间的电子转移,导致荧光猝灭现象。此外,我们开发了一种带有 QDs 作为荧光探针的便携式 RGB 感应小工具,用于 Hg 检测,其线性范围为 5 至 200nM。此外,该小工具还用于自来水中 Hg 的检测。结果表明,实际自来水中的平均回收率在 97.2%至 115.3%之间,表明准确度和精密度满足实际应用要求。

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