从红海采集的特定海洋生物的抗增殖作用。

Antiproliferative effects of selected marine organisms collected from Red Sea.

作者信息

Abdel-Lateff Ahmed, Alarif Walied M, Al-Lihaibi Sultan S, Abdel-Naim Ashraf B

机构信息

Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, Jeddah, Saudi Arabia /Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt.

Department of Marine Chemistry, Faculty of Marine Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.

出版信息

Pak J Pharm Sci. 2017 Mar;30(2):381-386.

PMID:28649060

Abstract

Ten selected marine organisms representing different classes of marine fauna and flora were collected from Saudi Arabia territorial water. They were Antipathes dichotoma, Rumphella sp., Dictoyota dichotoma, Hyrtios erectus, Petrosia sp., Heteroxenia fuscescens, Rumphella aggregata, Sinularia polydactyla, Sarcophyton glaucum, Sarcophyton trocheliophorum. Samples were lyophilized and extracted. Their cytotoxic activity was assessed by determining their IC50's against HepG2, A549 and PC-3 cancer cell lines. The extracts showed variable activities against A549 with IC50 in the range 388.3-0.1µg/mL; HepG2 with IC50 in range 382.5-0.1μg/ml and PC-3 with IC50 in the range 428.6-0.1µg/mL. Dictoyota dichotoma, Hyrtios erectus, Rumphella aggregata and Sarcophyton glaucum exhibited the highest antiproliferative activity. Therefore, their impact on cell cycle was examined by flow cytometry technique. It was concluded that they cause G0/G1, S-phase and G2/M cell cycle arrest.

摘要

从沙特阿拉伯领海收集了十种选定的海洋生物，它们代表了不同类别的海洋动植物。它们是二叉黑珊瑚、朗氏软珊瑚属物种、二叉网地藻、直立多孔螅、肉芝软珊瑚属物种、褐色异柳珊瑚、聚合朗氏软珊瑚、多指叉珊藻、灰肉参、指状肉参。样本经冻干和提取后，通过测定它们对肝癌细胞系HepG2、肺癌细胞系A549和前列腺癌细胞系PC-3的半数抑制浓度（IC50）来评估其细胞毒性活性。提取物对A549显示出不同程度的活性，IC50范围为388.3至0.1µg/mL；对HepG2的IC50范围为382.5至0.1μg/ml，对PC-3的IC50范围为428.6至0.1µg/mL。二叉网地藻、直立多孔螅、聚合朗氏软珊瑚和灰肉参表现出最高的抗增殖活性。因此，通过流式细胞术技术检测了它们对细胞周期的影响。得出的结论是，它们会导致G0/G1期、S期和G2/M期细胞周期停滞。