Department of Biochemistry and Molecular Biology, Faculty of Basic Medical Science, Kunming Medical University, Kunming, Yunnan, China.
Ethic Drug Screening & Pharmacology Center, Key Laboratory of Chemistry in Ethnic Medicine Resource, State Ethnic Affairs Commission & Ministry of Education, Yunnan MinZu University, Kunming, Yunnan, China.
Chem Biol Drug Des. 2018 Jan;91(1):126-136. doi: 10.1111/cbdd.13063. Epub 2017 Jul 17.
The healing of chronic wounds remains a considerable challenge in clinical trials and imposes severe financial and physiological burdens on patients. Many works are being tried to find ideal clinical promoting wound healing biomaterials. Small bioactive peptides with low cost and easy production, store and transfer become excellent candidates. Here, we identified a novel peptide (named OM-LV20) from skin secretions of odorous frog Odorrana margaretae. The peptide had an amino acid sequence of "LVGKLLKGAVGDVCGLLPIC," contained an intramolecular disulfide bridge at the C-terminus, and was produced by post-translational processing of a 71-residue prepropeptide. Our results showed that OM-LV20 had no direct microbe-killing effects, hemolytic activity, or acute toxicity, but did exhibit weak antioxidant activity. OM-LV20 promoted wound healing against human keratinocytes (HaCaT) and human skin fibroblasts (HSF) in both time- and dose-dependent manners. In addition, it induced the proliferation of HaCaT but not HSF cells. Of note, OM-LV20 showed strong wound healing-promoting activity in a mice model of full-thickness skin wound. Our research indicates the cellular and animal level wound healing potential of OM-LV20, and thus provides a novel bioactive peptide template for the development of wound healing agents and medicine.
慢性伤口的愈合仍然是临床试验中的一个重大挑战，给患者带来了严重的经济和生理负担。许多工作都在尝试寻找理想的临床促进伤口愈合的生物材料。具有低成本、易于生产、储存和转运的小生物活性肽成为优秀的候选物。在这里，我们从臭蛙 Odorrana margaretae 的皮肤分泌物中鉴定出一种新型肽（命名为 OM-LV20）。该肽的氨基酸序列为“LVGKLLKGAVGDVCGLLPIC”，在 C 末端含有一个分子内二硫键，由 71 个残基的前肽经翻译后加工而成。我们的结果表明，OM-LV20 没有直接的杀菌作用、溶血活性或急性毒性，但表现出较弱的抗氧化活性。OM-LV20 以时间和剂量依赖的方式促进人角质形成细胞（HaCaT）和人皮肤成纤维细胞（HSF）的伤口愈合。此外，它诱导 HaCaT 细胞而非 HSF 细胞的增殖。值得注意的是，OM-LV20 在全层皮肤伤口的小鼠模型中表现出很强的促进伤口愈合的活性。我们的研究表明了 OM-LV20 在细胞和动物水平的伤口愈合潜力，为开发伤口愈合剂和药物提供了一种新的生物活性肽模板。
