da Costa Cristiane Aguiar, Ognibene Dayane Teixeira, Cordeiro Viviane Silva Cristino, de Bem Graziele Freitas, Santos Izabelle Barcellos, Soares Ricardo Andrade, de Melo Cunha Letícia L, Carvalho Lenize C R M, de Moura Roberto Soares, Resende Angela Castro
Department of Pharmacology, Institute of Biology, Rio de Janeiro State University , Rio de Janeiro, Brazil .
J Med Food. 2017 Oct;20(10):1002-1010. doi: 10.1089/jmf.2017.0011. Epub 2017 Jun 26.
Previously, we have demonstrated that the seeds of Euterpe oleracia Mart. (açaí) are rich in polyphenols with antihypertensive and antioxidant properties. This study evaluated the renal protective effects of the hydroalcoholic extract obtained from the seeds of açaí (ASE) fruits in two-kidney, one-clip (2K1C) renovascular hypertension. Young male Wistar rats were used to obtain 2K1C and sham groups. Animals received ASE (200 mg/(kg·day) in drinking water) or vehicle for 40 days. We evaluated serum and urinary parameters, renal structural changes, and oxidative status. The increase in systolic blood pressure of the 2K1C group was accompanied by a decrease in left kidney volume and number of glomeruli, as well as an increase in glomerular volume and collagen deposition. ASE prevented the alterations of these parameters, except the reduced kidney volume. Serum levels of urea and creatinine and urinary protein excretion were increased in the 2K1C group and treatment with ASE improved all these functional parameters. The increased oxidative damage in the 2K1C group, assessed by lipid and protein oxidation, was prevented by ASE. The nitrite content and both expression and activity of antioxidant enzymes (superoxide dismutase-1, catalase, and glutathione peroxidase) were lower in the 2K1C group and restored by ASE. ASE substantially reduced renal injury and prevented renal dysfunction in 2K1C rats probably through its antihypertensive and antioxidant effects, providing a natural resource for treatment and prevention of renovascular hypertension-related abnormalities.
此前，我们已经证明，巴西莓（Euterpe oleracia Mart.）的种子富含具有降压和抗氧化特性的多酚。本研究评估了从巴西莓果实种子中提取的水醇提取物（ASE）对二肾一夹（2K1C）肾血管性高血压的肾脏保护作用。使用年轻雄性Wistar大鼠建立2K1C组和假手术组。动物在40天内饮用含ASE（200 mg/(kg·天)）的水或溶剂。我们评估了血清和尿液参数、肾脏结构变化以及氧化状态。2K1C组收缩压升高的同时，左肾体积和肾小球数量减少，肾小球体积和胶原沉积增加。ASE可预防这些参数的改变，但肾脏体积减小除外。2K1C组血清尿素和肌酐水平以及尿蛋白排泄增加，ASE治疗改善了所有这些功能参数。ASE可预防通过脂质和蛋白质氧化评估的2K1C组氧化损伤增加。2K1C组亚硝酸盐含量以及抗氧化酶（超氧化物歧化酶-1、过氧化氢酶和谷胱甘肽过氧化物酶）的表达和活性较低，而ASE可使其恢复。ASE可能通过其降压和抗氧化作用显著减轻2K1C大鼠的肾损伤并预防肾功能障碍，为治疗和预防肾血管性高血压相关异常提供了一种天然资源。
