Department of Developmental and Stem Cell Biology, Beckman Research Institute of City of Hope, 1500 East Duarte Road, Duarte, CA, USA.
Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope, 1500 East Duarte Road, Duarte, CA, USA.
Cereb Cortex. 2018 Aug 1;28(8):2810-2816. doi: 10.1093/cercor/bhx160.
Alternative pre-mRNA splicing (AS) produces multiple isoforms of mRNAs and proteins from a single gene. It is most prevalent in the mammalian brain and is thought to contribute to the formation and/or maintenance of functional complexity of the brain. Increasing evidence has documented the significant changes of AS between different regions or different developmental stages of the brain, however, the dynamics of AS and the possible function of it during neural progenitor cell (NPC) differentiation is less well known. Here, using purified NPCs and their progeny neurons isolated from the embryonic mouse cerebral cortex, we characterized the global differences of AS events between the 2 cell types by deep sequencing. The sequencing results revealed cell type-specific AS in NPCs and neurons that are important for distinct functions pertinent to the corresponding cell type. Our data may serve as a resource useful for further understanding how AS contributes to molecular regulations in NPCs and neurons during cortical development.
选择性剪接（AS）能从单个基因中产生多种 mRNA 和蛋白质的异构体。它在哺乳动物大脑中最为普遍，被认为有助于大脑的形成和/或功能复杂性的维持。越来越多的证据表明，大脑的不同区域或不同发育阶段之间的 AS 存在显著变化，然而，神经祖细胞（NPC）分化过程中 AS 的动态变化及其可能的功能还知之甚少。在这里，我们使用从胚胎小鼠大脑皮层中分离出的纯化 NPC 及其祖细胞神经元，通过深度测序来描述这 2 种细胞类型之间 AS 事件的全局差异。测序结果揭示了 NPC 和神经元中细胞类型特异性的 AS，这些 AS 对于与相应细胞类型相关的特定功能非常重要。我们的数据可以作为一种资源，有助于进一步了解 AS 如何在皮质发育过程中对 NPC 和神经元中的分子调控做出贡献。
