Rocha M, Vázquez M, Garciarrubio A, Covarrubias A A
Gene. 1985;37(1-3):91-9. doi: 10.1016/0378-1119(85)90261-6.
The nucleotide (nt) sequence of a 682-bp fragment containing the 3' end of the glnA gene, the region between the glnA and glnL genes, and the 5' end of the glnL gene from Escherichia coli was determined. This segment contains the region coding for the last 107 amino acids (aa) of glutamine synthetase, including the adenylylation site of this enzyme. The analysis of this sequence revealed two REP sequences, a Rho-independent terminator, the putative glnL promoter and the possible binding site for the glnG product, NRI.
测定了来自大肠杆菌的一段682bp片段的核苷酸(nt)序列,该片段包含谷氨酰胺合成酶基因(glnA)的3′末端、glnA基因与glnL基因之间的区域以及glnL基因的5′末端。该片段包含编码谷氨酰胺合成酶最后107个氨基酸(aa)的区域,包括该酶的腺苷酸化位点。对该序列的分析揭示了两个重复外显子回文序列(REP序列)、一个不依赖于Rho因子的终止子、假定的glnL启动子以及glnG产物NRI的可能结合位点。