在无蛋黄胰蛋白胨亚硫酸盐环丝氨酸琼脂中对食源性产气荚膜梭菌进行计数。
Enumeration of food-borne Clostridium perfringens in egg yolk-free tryptose-sulfite-cycloserine agar.
作者信息
Hauschild A H, Hilsheimer R
出版信息
Appl Microbiol. 1974 Mar;27(3):521-6. doi: 10.1128/am.27.3.521-526.1974.
The SFP (Shahidi-Ferguson perfringens), TSC (tryptose-sulfite-cycloserine), EY (egg yolk)-free TSC, and OPSP (oleandomycin-polymyxin-sulfadiazine perfringens) agars have been tested for their suitability to enumerate Clostridium perfringens in naturally contaminated foods. Complete recoveries of C. perfringens were obtained in each of the four media, but only the TSC and EY-free TSC agars were sufficiently selective to ensure subsequent confirmatory tests without interference from facultative anaerobes. Because of some disadvantages associated with the use of egg yolk, EY-free TSC agar is recommended for enumeration of C. perfringens in foods. Several conditions for convenient shipment of foods and C. perfringens isolates with minimum loss of viability have been tested. The highest viable counts were preserved when foods were mixed 1:1 (wt/vol) with 20% glycerol and kept in a container with dry ice. Isolated C. perfringens strains remained viable for at least 2 weeks at ambient temperatures on blood agar slopes with a 2% agar overlay in screw-cap culture tubes.
已对沙希迪-弗格森产气荚膜梭菌（SFP）琼脂、胰蛋白胨-亚硫酸盐-环丝氨酸（TSC）琼脂、无蛋黄（EY）的TSC琼脂以及竹桃霉素-多粘菌素-磺胺嘧啶产气荚膜梭菌（OPSP）琼脂检测了其在天然污染食品中对产气荚膜梭菌进行计数的适用性。在这四种培养基中均获得了产气荚膜梭菌的完全回收率，但只有TSC琼脂和无蛋黄的TSC琼脂具有足够的选择性，可确保后续确证试验不受兼性厌氧菌的干扰。由于使用蛋黄存在一些缺点，因此推荐使用无蛋黄的TSC琼脂对食品中的产气荚膜梭菌进行计数。已对食品和气荚膜梭菌分离株在运输过程中方便运输且活力损失最小的几种条件进行了测试。当食品与20%甘油按1:1（重量/体积）混合并保存在装有干冰的容器中时，可保持最高的活菌数。分离出的产气荚膜梭菌菌株在室温下于螺口培养管中含2%琼脂覆盖层的血琼脂斜面上至少可存活2周。
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