大肠杆菌独特的rpoA突变（phs）的表型特性。
Phenotypic properties of a unique rpoA mutation (phs) of Escherichia coli.
作者信息
Giffard P M, Rowland G C, Kroll R G, Stewart L M, Bakker E P, Booth I R
出版信息
J Bacteriol. 1985 Nov;164(2):904-10. doi: 10.1128/jb.164.2.904-910.1985.
Abstract
The phs mutation of Escherichia coli has been suggested to affect the Na+/H+ antiport (D. Zilberstein, E. Padan, and S. Schuldiner, FEBS Lett. 168:327-330, 1980). We have recently shown that the mutation affects the rpoA gene and thus affects transcription. The extent of the pleiotropy of the phs mutation was investigated. In addition to the previously reported growth defect on L-glutamate and melibiose, the mutation also affects at least two other metabolic systems. The transport and metabolism of arabinose is impaired and the transport of sulfate is reduced. The extent to which the effects of the phs mutation on metabolism are due to a defect in the Na+/H+ antiport was investigated, and no causal role for this transport system in the metabolic defects was found.
摘要
有人提出，大肠杆菌的phs突变会影响Na⁺/H⁺逆向转运（D. 齐尔伯斯坦、E. 帕丹和S. 舒尔迪纳，《欧洲生物化学学会联合会快报》168:327 - 330，1980年）。我们最近发现，该突变影响rpoA基因，从而影响转录。我们对phs突变的多效性程度进行了研究。除了先前报道的在L - 谷氨酸和蜜二糖上的生长缺陷外，该突变还至少影响另外两个代谢系统。阿拉伯糖的转运和代谢受损，硫酸盐的转运减少。我们研究了phs突变对代谢的影响在多大程度上是由于Na⁺/H⁺逆向转运缺陷所致，结果发现该转运系统在代谢缺陷中没有因果作用。
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