Joanne Sylvia, Vythilingam Indra, Teoh Boon-Teong, Leong Cherng-Shii, Tan Kim-Kee, Wong Meng-Li, Yugavathy Nava, AbuBakar Sazaly
Department of Parasitology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Department of Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Trop Med Int Health. 2017 Sep;22(9):1154-1165. doi: 10.1111/tmi.12918. Epub 2017 Jul 17.
To determine the susceptibility status of Aedes albopictus with and without Wolbachia to the four dengue virus serotypes.
Two newly colonised colonies of Ae. albopictus from the wild were used for the study. One colony was naturally infected with Wolbachia while in the other Wolbachia was removed by tetracycline treatment. Both colonies were orally infected with dengue virus-infected fresh blood meal. Dengue virus load was measured using quantitative RT-PCR at four-time intervals in the salivary glands, midguts and ovaries.
Wolbachia did not significantly affect Malaysian Ae. albopictus dengue infection or the dissemination rate for all four dengue virus serotypes. Malaysian Ae. albopictus had the highest replication kinetics for DENV-1 and the highest salivary gland and midgut infection rate for DENV-4.
Wolbachia, which naturally exists in Malaysian Ae. albopictus, does not significantly affect dengue virus replication. Malaysian Ae. albopictus is susceptible to dengue virus infections and capable of transmitting dengue virus, especially DENV-1 and DENV-4. Removal of Wolbachia from Malaysian Ae. albopictus would not reduce their susceptibility status.
确定携带和不携带沃尔巴克氏体的白纹伊蚊对四种登革病毒血清型的易感性状况。
使用两个新从野外采集并建立的白纹伊蚊种群进行研究。一个种群自然感染了沃尔巴克氏体，而另一个种群通过四环素处理去除了沃尔巴克氏体。两个种群均经口感染含有登革病毒的新鲜血餐。在四个时间点，使用定量逆转录聚合酶链反应（qRT-PCR）检测唾液腺、中肠和卵巢中的登革病毒载量。
沃尔巴克氏体对马来西亚白纹伊蚊感染登革病毒以及对所有四种登革病毒血清型的传播率均无显著影响。马来西亚白纹伊蚊对登革病毒1型（DENV-1）具有最高的复制动力学，对登革病毒4型（DENV-4）具有最高的唾液腺和中肠感染率。
自然存在于马来西亚白纹伊蚊中的沃尔巴克氏体对登革病毒复制无显著影响。马来西亚白纹伊蚊易感染登革病毒且能够传播登革病毒，尤其是DENV-1和DENV-4。从马来西亚白纹伊蚊中去除沃尔巴克氏体不会降低它们的易感性状况。
