Nakamura Risa, Okura Tsuyoshi, Fujioka Yohei, Sumi Keisuke, Matsuzawa Kazuhiko, Izawa Shoichiro, Ueta Etsuko, Kato Masahiko, Taniguchi Shin-Ichi, Yamamoto Kazuhiro
Division of Cardiovascular Medicine, Endocrinology and Metabolism, Department of Molecular Medicine and Therapeutics, Tottori University Faculty of Medicine, Yonago, Tottori, Japan.
Department of Regional Medicine, Tottori University Faculty of Medicine, Yonago, Tottori, Japan.
PLoS One. 2017 Jun 27;12(6):e0179737. doi: 10.1371/journal.pone.0179737. eCollection 2017.
Type 2 diabetes mellitus (T2DM) is caused by insulin resistance and β cell dysfunction. In recent studies reported that several markers associated with insulin sensitivity in skeletal muscle, Adiponectin and other parameters, such as fatty acid-binding protein (FABP4), have been reported to regulate insulin resistance, but it remains unclear which factor mostly affects insulin resistance in T2DM. In this cross-sectional study, we evaluated the relationships between several kinds of biomarkers and insulin resistance, and insulin secretion in T2DM and healthy controls. We recruited 30 participants (12 T2DM and 18 non-diabetic healthy controls). Participants underwent a meal tolerance test during which plasma glucose, insulin and serum C-peptide immunoreactivity were measured. We performed a hyperinsulinemic-euglycemic clamp and measured the glucose-disposal rate (GDR). The fasting serum levels of adiponectin, insulin-like growth factor-1, irisin, autotaxin, FABP4 and interleukin-6 were measured by ELISA. We found a strong negative correlation between FABP4 concentration and GDR in T2DM (r = -0.657, p = 0.020). FABP4 also was positively correlated with insulin secretion during the meal tolerance test in T2DM (IRI (120): r = 0.604, p = 0.038) and was positively related to the insulinogenic index in non-DM subjects (r = 0.536, p = 0.022). Autotaxin was also related to GDR. However, there was no relationship with insulin secretion. We found that serum FABP4 concentration were associated with insulin resistance and secretion in T2DM. This suggests that FABP4 may play an important role in glucose homeostasis.
2型糖尿病（T2DM）由胰岛素抵抗和β细胞功能障碍引起。最近的研究报道，骨骼肌中与胰岛素敏感性相关的几种标志物、脂联素以及其他参数，如脂肪酸结合蛋白（FABP4），已被报道可调节胰岛素抵抗，但在T2DM中哪种因素对胰岛素抵抗的影响最大仍不清楚。在这项横断面研究中，我们评估了T2DM患者和健康对照者中几种生物标志物与胰岛素抵抗及胰岛素分泌之间的关系。我们招募了30名参与者（12名T2DM患者和18名非糖尿病健康对照者）。参与者接受了糖耐量试验，在此期间测量血浆葡萄糖、胰岛素和血清C肽免疫反应性。我们进行了高胰岛素-正血糖钳夹试验并测量了葡萄糖处置率（GDR）。通过酶联免疫吸附测定法测量空腹血清脂联素、胰岛素样生长因子-1、鸢尾素、自分泌运动因子、FABP4和白细胞介素-6的水平。我们发现T2DM患者中FABP4浓度与GDR之间存在强负相关（r = -0.657，p = 0.020）。在T2DM患者的糖耐量试验期间，FABP4也与胰岛素分泌呈正相关（IRI（120）：r = 0.604，p = 0.038），并且在非糖尿病受试者中与胰岛素生成指数呈正相关（r = 0.536，p = 0.022）。自分泌运动因子也与GDR有关。然而，与胰岛素分泌无关。我们发现血清FABP4浓度与T2DM患者的胰岛素抵抗和分泌有关。这表明FABP4可能在葡萄糖稳态中起重要作用。
