Barochia Amisha V, Gordon Elizabeth M, Kaler Maryann, Cuento Rosemarie A, Theard Patricia, Figueroa Debbie M, Yao Xianglan, Weir Nargues A, Sampson Maureen L, Stylianou Mario, Choy David F, Holweg Cecile T J, Remaley Alan T, Levine Stewart J
Laboratory of Asthma and Lung Inflammation, National Institutes of Health, Bethesda, MD 20892.
Laboratory of Asthma and Lung Inflammation, National Institutes of Health, Bethesda, MD 20892.
J Lipid Res. 2017 Aug;58(8):1713-1721. doi: 10.1194/jlr.P077776. Epub 2017 Jun 27.
Blood eosinophil counts and serum periostin levels are biomarkers of type 2 inflammation. Although serum levels of HDL and apoA-I have been associated with less severe airflow obstruction in asthma, it is not known whether serum lipids or lipoprotein particles are correlated with type 2 inflammation in asthmatics. Here, we assessed whether serum lipids and lipoproteins correlated with blood eosinophil counts or serum periostin levels in 165 atopic asthmatics and 163 nonasthmatic subjects with and without atopy. Serum lipids and lipoproteins were quantified using standard laboratory assays and NMR spectroscopy. Absolute blood eosinophils were quantified by complete blood counts. Periostin levels were measured using the Elecsys® periostin assay. In atopic asthmatics, blood eosinophils negatively correlated with serum HDL cholesterol and total HDL particles measured by NMR spectroscopy (HDL). Serum periostin levels negatively correlated with total HDL In contrast, blood eosinophil counts positively correlated with serum triglyceride levels. This study demonstrates for the first time that HDL particles were negatively correlated, whereas serum triglycerides were positively correlated, with blood eosinophils in atopic asthmatics. This supports the concept that serum levels of HDL and triglycerides may be linked to systemic type 2 inflammation in atopic asthma.
血液嗜酸性粒细胞计数和血清骨膜蛋白水平是2型炎症的生物标志物。虽然血清高密度脂蛋白（HDL）和载脂蛋白A-I（apoA-I）水平与哮喘中较轻的气流阻塞有关，但尚不清楚血清脂质或脂蛋白颗粒是否与哮喘患者的2型炎症相关。在此，我们评估了165名特应性哮喘患者和163名有或无特应性的非哮喘受试者的血清脂质和脂蛋白是否与血液嗜酸性粒细胞计数或血清骨膜蛋白水平相关。血清脂质和脂蛋白通过标准实验室检测和核磁共振波谱法进行定量。绝对血液嗜酸性粒细胞通过全血细胞计数进行定量。骨膜蛋白水平使用Elecsys®骨膜蛋白检测法进行测量。在特应性哮喘患者中，血液嗜酸性粒细胞与通过核磁共振波谱法测量的血清HDL胆固醇和总HDL颗粒（HDL）呈负相关。血清骨膜蛋白水平与总HDL呈负相关。相比之下，血液嗜酸性粒细胞计数与血清甘油三酯水平呈正相关。本研究首次表明，在特应性哮喘患者中，HDL颗粒与血液嗜酸性粒细胞呈负相关，而血清甘油三酯与血液嗜酸性粒细胞呈正相关。这支持了HDL和甘油三酯的血清水平可能与特应性哮喘中的全身性2型炎症相关的概念。
