Unesco Chinese Center of Marine Biotechnology, Ocean University of China, Qingdao, China.
Bioresour Technol. 2012 Nov;124:77-82. doi: 10.1016/j.biortech.2012.08.024. Epub 2012 Aug 14.
In this study, an inulinase-producing yeast strain Pcla22 of Pichia guilliermondii was identified. It was found that the yeast strain Pcla22 could produce higher amount of oil and more lipid bodies in its cells than any other yeast strains tested in this study. Under the optimal conditions, 60.6%(w/w) of lipid based on cell dry weight, 20.4 g/l of the dry cell mass, SCO produced per g of consumed sugar of 0.19 g/g and biomass produced per g of consumed sugar of 0.32 g/g were obtained in the culture of the yeast strain Pcla22 after 96 h of the fed-batch fermentation. Over 79.8% of the fatty acids from the yeast strain Pcla22 grown in the oil production medium containing inulin was C(16:0) and C(18:1), especially C(18:1) (57.9%). The biodiesel obtained from the produced lipid could be burnt well.
在这项研究中，鉴定出一株产菊粉酶的毕赤酵母 Pcla22。研究发现，与本研究中测试的其他酵母菌株相比，酵母菌株 Pcla22 能够在其细胞中产生更高量的油和更多的脂滴。在最佳条件下，酵母菌株 Pcla22 在补料分批发酵 96 小时后，基于细胞干重的 60.6%(w/w)的脂质、20.4g/l 的干细胞质量、每消耗 1g 糖产生的 SCO 为 0.19g/g 和每消耗 1g 糖产生的生物质为 0.32g/g。在含有菊粉的产油培养基中生长的酵母菌株 Pcla22 中，超过 79.8%的脂肪酸为 C(16:0)和 C(18:1)，特别是 C(18:1) (57.9%)。从所产脂质获得的生物柴油燃烧性能良好。
