Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Department of Analytical Chemistry, Complutense University of Madrid , Madrid E-28040, Spain.
Nano Lett. 2017 Aug 9;17(8):4958-4963. doi: 10.1021/acs.nanolett.7b02102. Epub 2017 Jul 10.
Nanoparticles composed of Prussian Blue, PB, and the cyanometalate structural analogues, CuFe, FeCoFe, and FeCo, are examined as inorganic clusters that mimic the functions of peroxidases. PB acts as a superior catalyst for the oxidation of dopamine to aminochrome by HO. The oxidation of dopamine by HO in the presence of PB is 6-fold faster than in the presence of CuFe. The cluster FeCo does not catalyze the oxidation of dopamine to aminochrome. The most efficient catalyst for the generation of chemiluminescence by the oxidation of luminol by HO is, however, FeCo, and PB lacks any catalytic activity toward the generation of chemiluminescence. The order of catalyzed chemiluminescence generation is FeCo ≫ CuFe > FeCoFe. The clusters PB, CuFe, FeCoFe, and FeCo mimic the functions of NADH peroxidase. The catalyzed oxidation of NADH by HO to form NAD follows the order PB ≫ CuFe ∼ FeCoFe, FeCo. The efficient generation of chemiluminescence by the FeCo-catalyzed oxidation of luminol by HO is used to develop a glucose sensor. The aerobic oxidation of glucose in the presence of glucose oxidase, GOx, yields gluconic acid and HO. The chemiluminescence intensities formed by the GOx-generated HO relate to the concentration of glucose, thus providing a quantitative readout signal for the concentrations of glucose.
由普鲁士蓝 (PB) 以及氰合金属结构类似物 CuFe、FeCoFe 和 FeCo 组成的纳米颗粒被视为模拟过氧化物酶功能的无机簇。PB 作为 HO 氧化多巴胺生成氨基胆色素的优越催化剂。在 PB 存在下，HO 氧化多巴胺的速度比在 CuFe 存在下快 6 倍。簇 FeCo 不能催化多巴胺氧化生成氨基胆色素。然而，HO 氧化鲁米诺产生化学发光的最有效催化剂是 FeCo，而 PB 对化学发光的产生没有任何催化活性。通过 HO 氧化鲁米诺生成化学发光的催化活性顺序为 FeCo ≫ CuFe > FeCoFe。PB、CuFe、FeCoFe 和 FeCo 模拟 NADH 过氧化物酶的功能。HO 催化 NADH 氧化生成 NAD 的顺序为 PB ≫ CuFe ∼ FeCoFe, FeCo。HO 催化 FeCo 氧化鲁米诺产生的化学发光的高效生成被用于开发葡萄糖传感器。在葡萄糖氧化酶 (GOx) 的存在下，葡萄糖的有氧氧化生成葡萄糖酸和 HO。GOx 产生的 HO 形成的化学发光强度与葡萄糖的浓度有关，因此为葡萄糖的浓度提供了定量读出信号。
