Dadu Rama H R, Ford Rebecca, Sambasivam Prabhakaran, Gupta Dorin
Faculty of Veterinary and Agricultural Sciences, University of MelbourneDookie, VIC, Australia.
Environmental Futures Research Institute, School of Natural Sciences, Griffith UniversityNathan, QLD, Australia.
Front Plant Sci. 2017 Jun 14;8:1038. doi: 10.3389/fpls.2017.01038. eCollection 2017.
Substantial yield losses and poor seed quality are frequently associated with Ascochyta blight infection of lentil caused by . Recently reported changes in aggressiveness of have led to decreased resistance within cultivars, such as Northfield and Nipper in Australia. Furthermore, the narrow genetic base of the current breeding program remains a risk for further selective pathogen evolution to overcome other currently used resistances. Therefore, incorporation of potentially novel and diverse resistance genes into the advanced lines will aid to improve cultivar stability. To identify these, 30 genotypes sourced from five wild species ( and ), including eight previously reported resistance sources, were screened for disease reaction to two recently isolated and highly aggressive isolates. Subsequently, two accessions were found highly resistant and a further six , one , one , one , and one accessions were moderately resistant. Several of these were more resistant than the currently deployed resistance source, ILL 7537. Furthermore, accession ILWL 180 was consistently resistant against other highly aggressive isolates recovered from diverse geographical lentil growing regions and host genotypes, suggesting stability and potential for future use of this accession in the Australian lentil breeding program.
产量大幅损失和种子质量不佳常常与由……引起的小扁豆壳二孢叶枯病感染有关。最近报道的……致病性变化导致了品种内抗性下降，如澳大利亚的诺斯菲尔德和尼珀品种。此外，当前育种计划的遗传基础狭窄，仍然存在病原体进一步选择性进化以克服其他当前使用的抗性的风险。因此，将潜在的新型和多样的抗性基因整合到高级品系中将有助于提高品种稳定性。为了识别这些基因，对来自五个野生种（……和……）的30个基因型进行了筛选，其中包括八个先前报道的抗性来源，以检测它们对两种最近分离出的高致病性分离株的病害反应。随后，发现两个……种质高度抗病，另外六个……、一个……、一个……、一个……和一个……种质中度抗病。其中几个比目前使用的抗性来源ILL 7537更抗病。此外，……种质ILWL 180对从不同地理区域的小扁豆种植区和寄主基因型中分离出的其他高致病性分离株始终具有抗性，这表明该种质具有稳定性，有潜力在澳大利亚小扁豆育种计划中用于未来育种。
