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通过对感染豌豆壳二孢菌（Ascochyta lentis）的 24 小时内的转录组进行分析，揭示了关键的防御反应基因。

Transcriptome profiling of lentil (Lens culinaris) through the first 24 hours of Ascochyta lentis infection reveals key defence response genes.

机构信息

Glycomics institute, School of Sciences, Griffith University, 58 Parklands Dr., Southport, Gold Coast, 4215, QLD, Australia.

Environmental Futures Research Institute, School of Natural Sciences, Griffith University, 170 Kessels Rd., Nathan, 4111, QLD, Australia.

出版信息

BMC Genomics. 2018 Jan 31;19(1):108. doi: 10.1186/s12864-018-4488-1.

DOI:10.1186/s12864-018-4488-1
PMID:29385986
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5793396/
Abstract

BACKGROUND

Ascochyta blight, caused by the fungus Ascochyta lentis, is one of the most destructive lentil diseases worldwide, resulting in over $16 million AUD annual loss in Australia alone. The use of resistant cultivars is currently considered the most effective and environmentally sustainable strategy to control this disease. However, little is known about the genes and molecular mechanisms underlying lentil resistance against A. lentis.

RESULTS

To uncover the genetic basis of lentil resistance to A. lentis, differentially expressed genes were profiled in lentil plants during the early stages of A. lentis infection. The resistant 'ILL7537' and susceptible 'ILL6002' lentil genotypes were examined at 2, 6, and 24 h post inoculation utilising high throughput RNA-Sequencing. Genotype and time-dependent differential expression analysis identified genes which play key roles in several functions of the defence response: fungal elicitors recognition and early signalling; structural response; biochemical response; transcription regulators; hypersensitive reaction and cell death; and systemic acquired resistance. Overall, the resistant genotype displayed an earlier and faster detection and signalling response to the A. lentis infection and demonstrated higher expression levels of structural defence-related genes.

CONCLUSIONS

This study presents a first-time defence-related transcriptome of lentil to A. lentis, including a comprehensive characterisation of the molecular mechanism through which defence against A. lentis is induced in the resistant lentil genotype.

摘要

背景

由真菌长喙壳菌引起的壳二孢叶斑病是世界范围内最具破坏性的小扁豆病害之一，仅在澳大利亚每年就造成超过 1600 万澳元的损失。目前，使用抗性品种被认为是控制这种疾病最有效和最具环境可持续性的策略。然而，人们对小扁豆对长喙壳菌的抗性的基因和分子机制知之甚少。

结果

为了揭示小扁豆对长喙壳菌抗性的遗传基础，在长喙壳菌感染的早期阶段对小扁豆植株中的差异表达基因进行了分析。利用高通量 RNA 测序，在接种后 2、6 和 24 小时检查了抗性 'ILL7537' 和敏感 'ILL6002' 小扁豆基因型。基因型和时间依赖性差异表达分析鉴定出了在防御反应的几个功能中起关键作用的基因：真菌激发子的识别和早期信号转导；结构反应；生化反应；转录调节剂；过敏反应和细胞死亡；以及系统获得性抗性。总的来说，抗性基因型对长喙壳菌感染表现出更早和更快的检测和信号反应，并表现出更高水平的结构防御相关基因的表达。

结论

本研究首次对小扁豆与长喙壳菌的防御相关转录组进行了研究，包括对抗性小扁豆基因型中诱导防御长喙壳菌的分子机制进行了全面的特征描述。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/ad963f9d3f85/12864_2018_4488_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/b5216e6aa549/12864_2018_4488_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/c72f13558340/12864_2018_4488_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/bbd7be7533f9/12864_2018_4488_Fig7_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/e7abdad679d9/12864_2018_4488_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/ad963f9d3f85/12864_2018_4488_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/b5216e6aa549/12864_2018_4488_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/056d5e24cca2/12864_2018_4488_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/ee83f9824a61/12864_2018_4488_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/bc3bc2ca1f3d/12864_2018_4488_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/e28378859a60/12864_2018_4488_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/c72f13558340/12864_2018_4488_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/bbd7be7533f9/12864_2018_4488_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/3b6db66fc552/12864_2018_4488_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/fd183f199e42/12864_2018_4488_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/e7abdad679d9/12864_2018_4488_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d920/5793396/ad963f9d3f85/12864_2018_4488_Fig11_HTML.jpg

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