嗜铁钩端螺旋菌的基因改良以增强对磷酸盐的生物去除能力
Genetic improvement of Magnetospirillum gryphiswaldense for enhanced biological removal of phosphate.
作者信息
Zhou Yanping, Lisowski Wiktor, Zhou Yan, Jern Ng Wun, Huang Kama, Fong Eileen
机构信息
College of Electronics and Information Engineering, Sichuan University, Chengdu, 610065, China.
School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore, 639798, Singapore.
出版信息
Biotechnol Lett. 2017 Oct;39(10):1509-1514. doi: 10.1007/s10529-017-2383-5. Epub 2017 Jun 28.
OBJECTIVES
To improve its phosphate accumulating abilities for phosphate recycling from wastewater, a magnetotactic bacterium, Magnetospirillum gryphiswaldense, was genetically modified to over-express polyphosphate kinase.
RESULTS
Polyphosphate kinase was over-expressed in the bacterium. The recombinant strain accumulated ninefold more polyphosphate from synthetic wastewater compared to original wild type. The magnetic property of the recombinant M. gryphiswaldense strain was retained.
CONCLUSIONS
The recombinant M. gryphiswaldense can be used for phosphate removal and recovery in bioremediation.
目的
为提高从废水中回收磷的聚磷能力，对趋磁细菌格氏嗜盐碱螺旋菌进行基因改造，使其过量表达多聚磷酸激酶。
结果
多聚磷酸激酶在该细菌中过量表达。与原始野生型相比，重组菌株从合成废水中积累的多聚磷酸盐多九倍。重组格氏嗜盐碱螺旋菌菌株的磁性得以保留。
结论
重组格氏嗜盐碱螺旋菌可用于生物修复中磷的去除和回收。
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