School of Ocean Science and Technology, Dalian University of Technology, No. 2 Dagong Road, Panjin, Liaoning 124221, China.
School of Ocean Science and Technology, Dalian University of Technology, No. 2 Dagong Road, Panjin, Liaoning 124221, China.
Mar Pollut Bull. 2020 Aug;157:111333. doi: 10.1016/j.marpolbul.2020.111333. Epub 2020 Jun 17.
Paralytic shellfish toxins (PSTs) cause risks to human health through food chains. Understanding the change of PSTs in seawater is critical for predicting the safety of seafood. Most reported methods for the detection of PSTs in microalgae or shellfish are not applicable in seawater because of extremely low concentration and matrix interferences. High resolution mass spectrometry (HRMS), quadrupole exactive orbitrap detects molecular ions accurately, and molecularly imprinted solid-phase extraction (MISPE) is recognized effective to reduce the matrix interference. GTXs 2&3 are two of common marine toxins in PSTs. In this study, a sensitive method consisting MISPE and liquid chromatography LC-HRMS was developed for the detection of GTXs 2&3 with a limit of detection (LOD) of 47.4 ng/L in seawater. With this method, samples obtained from the estuaries of the Shuangtaizi and Daliao Rivers were analyzed, and the results indicated the concentrations were lower than LOD in the area under investigation.
麻痹性贝类毒素（PSTs）通过食物链对人类健康造成危害。了解海水中 PSTs 的变化对于预测海鲜的安全性至关重要。由于浓度极低且基质干扰严重，大多数已报道的用于检测微藻或贝类中 PSTs 的方法在海水中并不适用。高分辨率质谱（HRMS）、四极精确轨道阱可以准确检测分子离子，分子印迹固相萃取（MISPE）被认为可以有效降低基质干扰。GTXs 2&3 是 PSTs 中两种常见的海洋毒素。在本研究中，建立了一种灵敏的方法，该方法包括 MISPE 和 LC-HRMS，用于检测海水中 GTXs 2&3 的含量，其检测限（LOD）为 47.4ng/L。使用该方法对双台子河和大辽河口的样品进行了分析，结果表明在所研究的区域内浓度均低于 LOD。
