Walters Sarah, Feeks James A, Huynh Khang T, Hunter Jennifer J
The Institute of Optics, University of Rochester, Rochester, NY 14642, USA.
Center for Visual Science, University of Rochester, Rochester, NY 14642, USA.
Biomed Opt Express. 2021 Dec 17;13(1):389-407. doi: 10.1364/BOE.444550. eCollection 2022 Jan 1.
Fluorescence lifetime imaging has demonstrated promise as a quantitative measure of cell health. Adaptive optics two-photon excited fluorescence (TPEF) ophthalmoscopy enables excitation of intrinsic retinal fluorophores involved in cellular metabolism and the visual cycle, providing visualization of retinal structure and function at the cellular scale. Combining these technologies revealed that macaque cones had a significantly longer mean TPEF lifetime than rods at 730 nm excitation. At 900 nm excitation, macaque photoreceptors had a significantly longer mean TPEF lifetime than the retinal pigment epithelium layer. AOFLIO can measure the fluorescence lifetime of intrinsic retinal fluorophores on a cellular scale, revealing differences in lifetime between retinal cell classes.
荧光寿命成像已展现出作为细胞健康定量指标的潜力。自适应光学双光子激发荧光（TPEF）检眼镜能够激发参与细胞代谢和视觉循环的视网膜内源性荧光团，在细胞尺度上实现视网膜结构和功能的可视化。将这两种技术相结合发现，在730纳米激发波长下，猕猴的视锥细胞平均TPEF寿命明显长于视杆细胞。在900纳米激发波长下，猕猴的光感受器平均TPEF寿命明显长于视网膜色素上皮层。AOFLIO能够在细胞尺度上测量视网膜内源性荧光团的荧光寿命，揭示不同视网膜细胞类型之间的寿命差异。
