Dysli Chantal, Berger Lieselotte, Wolf Sebastian, Zinkernagel Martin S
Departments of Ophthalmology and Clinical Research, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Retina. 2017 Nov;37(11):2151-2161. doi: 10.1097/IAE.0000000000001452.
To quantify retinal fluorescence lifetimes in patients with central serous chorioretinopathy (CSC) and to identify disease specific lifetime characteristics over the course of disease.
Forty-seven participants were included in this study. Patients with central serous chorioretinopathy were imaged with fundus photography, fundus autofluorescence, optical coherence tomography, and fluorescence lifetime imaging ophthalmoscopy (FLIO) and compared with age-matched controls. Retinal autofluorescence was excited using a 473-nm blue laser light and emitted fluorescence light was detected in 2 distinct wavelengths channels (498-560 nm and 560-720 nm). Clinical features, mean retinal autofluorescence lifetimes, autofluorescence intensity, and corresponding optical coherence tomography (OCT) images were further analyzed.
Thirty-five central serous chorioretinopathy patients with a mean visual acuity of 78 ETDRS letters (range, 50-90; mean Snellen equivalent: 20/32) and 12 age-matched controls were included. In the acute stage of central serous chorioretinopathy, retinal fluorescence lifetimes were shortened by 15% and 17% in the respective wavelength channels. Multiple linear regression analysis showed that fluorescence lifetimes were significantly influenced by the disease duration (P < 0.001) and accumulation of photoreceptor outer segments (P = 0.03) but independent of the presence or absence of subretinal fluid. Prolonged central macular autofluorescence lifetimes, particularly in eyes with retinal pigment epithelial atrophy, were associated with poor visual acuity.
This study establishes that autofluorescence lifetime changes occurring in central serous chorioretinopathy exhibit explicit patterns which can be used to estimate perturbations of the outer retinal layers with a high degree of statistical significance.
量化中心性浆液性脉络膜视网膜病变（CSC）患者的视网膜荧光寿命，并确定疾病过程中特定于该疾病的寿命特征。
本研究纳入了47名参与者。对中心性浆液性脉络膜视网膜病变患者进行眼底照相、眼底自发荧光、光学相干断层扫描和荧光寿命成像眼底镜检查（FLIO），并与年龄匹配的对照组进行比较。使用473nm蓝色激光激发视网膜自发荧光，并在2个不同的波长通道（498 - 560nm和560 - 720nm）检测发射的荧光。进一步分析临床特征、平均视网膜自发荧光寿命、自发荧光强度和相应的光学相干断层扫描（OCT）图像。
纳入了35名中心性浆液性脉络膜视网膜病变患者，平均视力为78个ETDRS字母（范围为50 - 90；平均Snellen等效值：20/32）和12名年龄匹配的对照组。在中心性浆液性脉络膜视网膜病变的急性期，相应波长通道的视网膜荧光寿命分别缩短了15%和17%。多元线性回归分析表明，荧光寿命受疾病持续时间（P < 0.001）和光感受器外段积累（P = 0.03）的显著影响，但与视网膜下液的有无无关。黄斑中心自发荧光寿命延长，特别是在伴有视网膜色素上皮萎缩的眼中，与视力差有关。
本研究表明，中心性浆液性脉络膜视网膜病变中发生的自发荧光寿命变化呈现出明确的模式，可用于以高度统计学意义估计视网膜外层的扰动。
