Rasulov Bakhtiyor A, Li Li, Liu Yong-Hong, Mohamad Osama Abdalla, Xiao Min, Ma Jin-Biao, Li Wen-Jun
Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürümqi, 830011, Xinjiang, People's Republic of China.
Institute of Genetics and Plant Experimental Biology, Uzbekistan Academy of Sciences, Kybray District, Tashkent, 111226, Uzbekistan.
3 Biotech. 2017 Jul;7(3):179. doi: 10.1007/s13205-017-0811-9. Epub 2017 Jun 29.
Synthesis of the exopolysaccharide-based bioflocculant Rhizobium radiobacter SZ4S7S14 was researched and medium optimized for enhanced production of bioflocculant. D-Mannose and yeast extract were found to be best carbon and nitrogen sources for maximal yield of bioflocculant. The bioflocculant samples obtained in different media with different carbon and nitrogen sources were further analyzed by SEM-EDX and FT-IR. FT-IR spectroscopy of the bioflocculant samples, obtained in different carbon/nitrogen sources showed slight modification of the structures of biopolymers. SEM micrographs revealed that exopolysaccharide-based bioflocculant of R. radiobacter SZ4S7S14 looks like bricks, and chemical structure of it can be varied due to utilized carbon and nitrogen source.
研究了基于胞外多糖的生物絮凝剂放射形土壤杆菌SZ4S7S14的合成,并对培养基进行了优化以提高生物絮凝剂的产量。发现D-甘露糖和酵母提取物是实现生物絮凝剂最大产量的最佳碳源和氮源。对在含有不同碳源和氮源的不同培养基中获得的生物絮凝剂样品进行了扫描电子显微镜-能谱分析(SEM-EDX)和傅里叶变换红外光谱分析(FT-IR)。在不同碳源/氮源中获得的生物絮凝剂样品的傅里叶变换红外光谱显示生物聚合物结构有轻微改变。扫描电子显微镜照片显示,放射形土壤杆菌SZ4S7S14的基于胞外多糖的生物絮凝剂看起来像砖块,并且其化学结构会因所使用的碳源和氮源而有所不同。