Zailer Elina, Holzgrabe Ulrike, Diehl Bernd W K
Spectral Service AG, Emil-Hoffmann-Strasse 33, 50996 Cologne, Germany; Julius-Maximilians-Universität, Institut für Pharmazie und Lebensmittelchemie, Am Hubland, 97074, Würzburg, Germany.
Julius-Maximilians-Universität, Institut für Pharmazie und Lebensmittelchemie, Am Hubland, 97074, Würzburg, Germany.
J AOAC Int. 2017 Nov 1;100(6):1819-1830. doi: 10.5740/jaoacint.17-0063. Epub 2017 Jun 29.
A proton (1H) NMR spectroscopic method was established for the quality assessment of vegetable oils. To date, several research studies have been published demonstrating the high potential of the NMR technique in lipid analysis. An interlaboratory comparison was organized with the following main objectives: (1) to evaluate an alternative analysis of edible oils by using 1H NMR spectroscopy; and (2) to determine the robustness and reproducibility of the method. Five different edible oil samples were analyzed by evaluating 15 signals (free fatty acids, peroxides, aldehydes, double bonds, and linoleic and linolenic acids) in each spectrum. A total of 21 NMR data sets were obtained from 17 international participant laboratories. The performance of each laboratory was assessed by their z-scores. The test was successfully passed by 90.5% of the participants. Results showed that NMR spectroscopy is a robust alternative method for edible oil analysis.
建立了一种用于植物油质量评估的质子（1H）核磁共振光谱法。迄今为止，已有多项研究发表，证明了核磁共振技术在脂质分析中的巨大潜力。组织了一次实验室间比对，主要目标如下：（1）评估使用1H核磁共振光谱法对食用油进行替代分析；（2）确定该方法的稳健性和重现性。通过评估每个光谱中的15个信号（游离脂肪酸、过氧化物、醛、双键以及亚油酸和亚麻酸）对5种不同的食用油样品进行了分析。共从17个国际参与实验室获得了21套核磁共振数据集。通过z分数评估每个实验室的表现。90.5%的参与者成功通过了测试。结果表明，核磁共振光谱法是一种用于食用油分析的稳健替代方法。
