Sultanpuram Vishnuvardhan Reddy, Mothe Thirumala, Chintalapati Sasikala, Chintalapati Venkata Ramana
Microbial Ecology Lab, Department of Biochemistry, Mahatma Gandhi University, Anneparthy, Yellareddygudem (PO), Nalgonda 508254, Telangana, India.
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J. N. T. University, Kukatpally, Hyderabad 500085, Telangana, India.
Int J Syst Evol Microbiol. 2017 Jun;67(6):1861-1866. doi: 10.1099/ijsem.0.001876.
A Gram-stain-positive, aerobic, non-motile, rod-shaped, non-endospore-forming bacterial strain, 10CT, was isolated from Lonar soda lake in India. Based on the 16S rRNA gene sequence analysis, this strain was identified as belonging to the genus Nesterenkonia and was most closely related to the type strains of Nesterenkonia lacusekhoensis (99.1 %, sequence similarity), Nesterenkonia aethiopica (96.9 %), Nesterenkonia flava (96.9 %) and related of the genus Nesterenkonia (<96.6 %, sequence similarity). However, the DNA-DNA relatedness of strain 10CT with N. lacusekhoensis KCTC 19283T was only 34.6±0.9. The DNA G+C content of strain 10CT was 68.6 mol%. Strain 10CT was an aerobic microbe with optimal growth at 37 °C, pH 7.5-8.0 and 5-6 % (w/v) NaCl. The cell-wall peptidoglycan of strain 10CT was of the type A4α (l-Lys-l-Glu). The major polar lipids present were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylcholine. The major isoprenoid quinones were MK-7, MK-8 and MK-9. Major fatty acids of strain 10CT were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The results of phylogenetic, chemotaxonomic and biochemical tests allowed a clear differentiation of strain 10CT, which represents a novel member of the genus Nesterenkonia for which the name Nesterenkonia cremea sp. nov. is proposed. The type strain is 10CT (=LMG 29100T=KCTC 39636T=CGMCC 1.15388T).
从印度洛纳尔盐湖分离出一株革兰氏阳性、需氧、不运动、杆状、不形成芽孢的细菌菌株10CT。基于16S rRNA基因序列分析，该菌株被鉴定为属于涅斯捷连科氏菌属，与涅斯捷连科氏湖生菌（99.1%，序列相似性）、涅斯捷连科氏埃塞俄比亚菌（96.9%）、涅斯捷连科氏黄色菌（96.9%）的模式菌株以及涅斯捷连科氏菌属的其他相关菌株（序列相似性<96.6%）关系最为密切。然而，菌株10CT与湖生涅斯捷连科氏菌KCTC 19283T的DNA-DNA相关性仅为34.6±0.9。菌株10CT的DNA G+C含量为68.6 mol%。菌株10CT是一种需氧微生物，在37°C、pH 7.5 - 8.0和5 - 6%（w/v）NaCl条件下生长最佳。菌株10CT的细胞壁肽聚糖为A4α型（l-赖氨酸-l-谷氨酸）。主要的极性脂质为磷脂酰甘油、双磷脂酰甘油和磷脂酰胆碱。主要的类异戊二烯醌为MK-7、MK-8和MK-9。菌株10CT的主要脂肪酸为anteiso-C15 : 0、anteiso-C17 : 0和iso-C16 : 0。系统发育、化学分类和生化测试结果使得菌株10CT得以明确区分，它代表了涅斯捷连科氏菌属的一个新成员，为此提议将其命名为涅斯捷连科氏乳黄色菌（Nesterenkonia cremea sp. nov.）。模式菌株为10CT（=LMG 29100T=KCTC 39636T=CGMCC 1.15388T）。
