Guangxi Minzu University, Nanning, China.
Yunnan University, Kunming, China.
Arch Microbiol. 2024 Feb 19;206(3):110. doi: 10.1007/s00203-023-03825-0.
A Gram-positive, aerobic, non-motile, irregular short rod, nonspore-forming actinobacterial strain, designated GX14115, was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0-3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4-45 °C (optimum 30 °C), at pH 4.5-10.0 (optimum pH 7.5) and in the presence of 0-12% NaCl (w/v) (optimum 3.5%). Chemotaxonomic analysis showed that the main menaquinone of strain GX14115 was MK-7. The major cellular fatty acids were anteiso-C (44.8%), anteiso-C (20.5%), and iso-C (16%). The whole-cell sugars were galactose and xylose. The peptidoglycan type was L-Lys-Gly-D-Asp, and the polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), one unknown phospholipid (UP), and one unknown glycolipid (UG). The DNA G + C content of the type of strain was 69.5 mol%. The 16S rRNA gene sequence analysis revealed that strain GX14115 is affiliated with the genus Nesterenkonia and is closely related to Nesterenkonia sandarakina YIM 70009 (96.5%) and Nesterenkonia lutea YIM 70081 (96.8%). The calculated results indicated that the average nucleotide identity (ANI) values of GX14115 were 74.49-74.78%, to the two aforementioned type strains, and the digital DNA-DNA hybridization (dDDH) values were 20.1-20.7%. Strain GX14115 was proposed as a novel species of the genus Nesterenkonia by the physiological, chemotaxonomic, and phylogenetic data, for whose the name is Nesterenkonia marinintestina sp. nov. The type of strain is GX14115 (= MCCC 1K06658 = KCTC 49495).
一株革兰氏阳性、需氧、非运动、不规则短杆、不产孢放线菌菌株，命名为 GX14115，从中国广西北海市的鱼类肠道中分离得到，并进行了多相分类学研究。在 30°C 下培养 72 小时后，在 2216E 培养基上，菌落呈黄色-绿色、圆形、光滑、中央凸起、凸起、不透明，直径为 2.0-3.0mm。生长在 4-45°C（最佳 30°C），pH 值 4.5-10.0（最佳 pH 值 7.5）和 0-12%NaCl（w/v）（最佳 3.5%）中。化学分类分析表明，菌株 GX14115 的主要menaquinone 为 MK-7。主要细胞脂肪酸为anteiso-C（44.8%）、anteiso-C（20.5%）和 iso-C（16%）。全细胞糖为半乳糖和木糖。肽聚糖类型为 L-Lys-Gly-D-Asp，极性脂为磷脂酰乙醇胺（PE）、双磷脂酰甘油（DPG）、一种未知磷脂（UP）和一种未知糖脂（UG）。模式菌株的 DNA G+C 含量为 69.5mol%。16S rRNA 基因序列分析表明，菌株 GX14115 属于内斯泰勒科尼亚属，与内斯泰勒科尼亚沙拉克纳亚 YIM 70009（96.5%）和内斯泰勒科尼亚 lutea YIM 70081（96.8%）密切相关。计算结果表明，GX14115 与上述两种模式株的平均核苷酸同一性（ANI）值为 74.49-74.78%，数字 DNA-DNA 杂交（dDDH）值为 20.1-20.7%。根据生理、化学分类和系统发育数据，菌株 GX14115 被提议为内斯泰勒科尼亚属的一个新种，其名称为 Nesterenkonia marinintestina sp. nov.。模式菌株为 GX14115（=MCCC 1K06658=KCTC 49495）。
