Park Sooyeon, Yoon Sun Young, Ha Min-Ji, Yoon Jung-Hoon
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jun;67(6):2036-2042. doi: 10.1099/ijsem.0.001919.
A Gram-stain-negative, aerobic, non-motile and ovoid or rod-shaped bacterial strain, designated OITF-11T, was isolated from a tidal flat in Oido, an island of South Korea, and subjected to a polyphasic taxonomic study. Strain OITF-11T grew optimally at 25 °C, at pH 7.0-8.0 and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OITF-11T belonged to the genus Polaribacter. Strain OITF-11T exhibited 16S rRNA gene sequence similarity values of 97.4-98.1 % to Polaribacter haliotis RA4-7T, Polaribacter atrinae KACC 17473T, Polaribacter dokdonensis DSW-5T and Polaribacter marinaquae KCTC 42664T, and of 94.1-96.9 % to the type strains of the other Polaribacter species. Strain OITF-11T contained menaquinone MK-6 as the predominant menaquinone and iso-C15 : 0, iso-C15 : 1 G, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH and C15 : 1ω6c as the major fatty acids. The major polar lipids detected in strain OITF-11T were phosphatidylethanolamine, phosphatidylmonomethylethanolamine and one unidentified lipid. The DNA G+C content of strain OITF-11T was 32.2 mol% and its DNA-DNA relatedness values with the type strains of P. haliotis, P. atrinae, P. dokdonensis and P. marinaquae were 14-33 %. Differential phenotypic properties, together with its phylogenetic and genetic distinctiveness, revealed that strain OITF-11T is separated from recognized species of the genus Polaribacter. On the basis of the data presented, strain OITF-11T is considered to represent a novel species of the genus Polaribacter, for which the name Polaribacter litorisediminis sp. nov. is proposed. The type strain is OITF-11T (=KCTC 52500T=NBRC 112457T).
从韩国岛屿巨济岛的潮滩中分离出一株革兰氏染色阴性、需氧、无运动性、卵形或杆状的细菌菌株，命名为OITF-11T，并对其进行了多相分类研究。菌株OITF-11T在25 °C、pH 7.0-8.0以及2.0 %（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列构建的系统发育树表明，菌株OITF-11T属于极地杆菌属。菌株OITF-11T与皱纹盘鲍极地杆菌RA4-7T、紫贻贝极地杆菌KACC 17473T、独岛极地杆菌DSW-5T和海洋极地杆菌KCTC 42664T的16S rRNA基因序列相似性值为97.4-98.1 %，与其他极地杆菌属模式菌株的相似性值为94.1-96.9 %。菌株OITF-11T以甲基萘醌MK-6作为主要甲基萘醌，以异-C15 : 0、异-C15 : 1 G、异-C15 : 0 3-OH、异-C17 : 0 3-OH和C15 : 1ω6c作为主要脂肪酸。在菌株OITF-11T中检测到的主要极性脂类为磷脂酰乙醇胺、磷脂酰单甲基乙醇胺和一种未鉴定的脂类。菌株OITF-11T的DNA G+C含量为32.2 mol%，其与皱纹盘鲍极地杆菌、紫贻贝极地杆菌、独岛极地杆菌和海洋极地杆菌模式菌株的DNA-DNA杂交值为14-33 %。不同的表型特征，以及其系统发育和遗传独特性，表明菌株OITF-11T与极地杆菌属已确认的物种不同。根据所提供的数据，菌株OITF-11T被认为代表了极地杆菌属的一个新物种，为此提出名称为海岸极地杆菌新种（Polaribacter litorisediminis sp. nov.）。模式菌株为OITF-11T（=KCTC 52500T=NBRC 112457T）。
