Wanasinghe W M Lakshani Anuradha, Xin Wang, Siliang Yuan, Qiu Dongru
National Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Curr Microbiol. 2025 Mar 12;82(5):186. doi: 10.1007/s00284-025-04166-5.
A Gram-stain-negative, strictly aerobic, rod-shaped, and no flagella, designated SLMDC-22, was isolated from the intestine of a mud crab (Scylla serrata). The molecular phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SLMDC-22 belonged to the genus Polaribacter and exhibited 96.9% similarity to P. huanghezhanensis, 96.5% to P. pacificus, 95.8% to P. lacunae, and 94.1% to P. marinivivus. The G + C content of the DNA of strain SLMDC-22 was 30.3%. Growth occurred at 4-34 °C (optimum, 23 °C), at pH 5-9 (optimum, pH 7.0), and with 0.5-2.0% (w/v) NaCl (optimum, 2%). The major fatty acid was iso-C. Major polar lipids include phospholipid, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, amino lipid, glycolipid, amino phospholipid, and an unknown polar lipid. The major respiratory quinone is MK-6. Based on whole-genome analysis, the orthologous average nucleotide identity (ANI) with the two closest relatives, Polaribacter huanghezhanensis and Polaribacter pacificus, were 87.3% and 75.7%, respectively. Furthermore, the digital DNA-DNA hybridization (dDDH) values against Polaribacter huanghezhanensis and Polaribacter pacificus were 75.8% and 19.6%, respectively. Notably, 25 genes of SLMDC-22 were associated with nitrogen metabolism, including denitrification, nitrate ammonification, ammonium assimilation, and nitrosative stress. The phenotypic, genotypic, phylogenetic, and chemotaxonomic data revealed that strain SLMDC-22 represents a novel species of the genus Polaribacter, for which the name Polaribacter uvawellassae sp. nov. is proposed. The type strain is SLMDC-22 (= KCTC 102289 = MCCC 1K09222).
从一只锯缘青蟹（Scylla serrata）的肠道中分离出一株革兰氏染色阴性、严格需氧、杆状且无鞭毛的菌株，命名为SLMDC - 22。基于16S rRNA基因序列的分子系统发育分析表明，菌株SLMDC - 22属于嗜冷杆菌属，与黄河站嗜冷杆菌（P. huanghezhanensis）的相似性为96.9%，与太平洋嗜冷杆菌（P. pacificus）的相似性为96.5%，与腔隙嗜冷杆菌（P. lacunae）的相似性为95.8%，与食海洋生物嗜冷杆菌（P. marinivivus）的相似性为94.1%。菌株SLMDC - 22的DNA的G + C含量为30.3%。在4 - 34°C（最适温度为23°C）、pH值为5 - 9（最适pH值为7.0）以及NaCl浓度为0.5 - 2.0%（w/v）（最适浓度为2%）的条件下可生长。主要脂肪酸为异戊酸。主要极性脂质包括磷脂、磷脂酰单甲基乙醇胺、磷脂酰乙醇胺、氨基脂质、糖脂、氨基磷脂和一种未知极性脂质。主要呼吸醌为MK - 6。基于全基因组分析，与两个亲缘关系最近的物种黄河站嗜冷杆菌和太平洋嗜冷杆菌的直系同源平均核苷酸同一性（ANI）分别为87.3%和75.7%。此外，与黄河站嗜冷杆菌和太平洋嗜冷杆菌的数字DNA - DNA杂交（dDDH）值分别为75.8%和19.6%。值得注意的是，SLMDC - 22的25个基因与氮代谢相关，包括反硝化作用、硝酸盐氨化作用、铵同化作用和亚硝化应激。表型、基因型、系统发育和化学分类学数据表明，菌株SLMDC - 22代表嗜冷杆菌属的一个新物种，为此提出名为极地沃氏嗜冷杆菌（Polaribacter uvawellassae sp. nov.）。模式菌株为SLMDC - 22（= KCTC 102289 = MCCC 1K09222）。
