Nian Si-Hui, Li Hui-Jun, Liu E-Hu, Li Ping
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 24 Tongjia Lane, Nanjing 210009, China.
J Pharm Biomed Anal. 2017 Oct 25;145:195-202. doi: 10.1016/j.jpba.2017.06.039. Epub 2017 Jun 19.
Comprehensive utilization of medicinal plant resources is of great significance for sustainable development of traditional Chinese medicines. In the present study, the α-glucosidase inhibitory activities of the rhizome and fibrous root of Anemarrhena Asphodeloides Bunge, were compared detailedly, and a high performance liquid chromatography coupled with electrospray ionization tandem triple quadrupole mass spectrometry (HPLC-QQQ/MS) method was developed for simultaneous quantification of seven bioactive constituents including neomangiferin, mangiferin, isomangiferin, timosaponin BII, timosaponin B, timosaponin AIII, and timosaponin N in 40 batches of samples. The results demonstrated that fibrous root extracts had more potent α-glucosidase inhibitory activity than rhizome extracts. Mangiferin and isomangiferin were abundant in fibrous root, while the analyzed saponins were rich in rhizome. Based on the chemometrics methods including principal component analysis (PCA), orthogonal partial least square discriminant analysis (OPLS-DA), and partial least square (PLS), mangiferin and isomangiferin might be mainly responsible for α-glucosidase inhibitory activity of the genus. These findings indicate that the established HPLC-QQQ/MS method was proven to be useful and efficient for quality control of Anemarrhena materials, and fibrous root had the potential to be utilized as anti-diabetic medicinal resource.
药用植物资源的综合利用对中药的可持续发展具有重要意义。在本研究中，详细比较了知母根茎和须根的α-葡萄糖苷酶抑制活性，并建立了一种高效液相色谱-电喷雾电离串联三重四极杆质谱联用（HPLC-QQQ/MS）方法，用于同时定量测定40批样品中的7种生物活性成分，包括新芒果苷、芒果苷、异芒果苷、知母皂苷BII、知母皂苷B、知母皂苷AIII和知母皂苷N。结果表明，须根提取物比根茎提取物具有更强的α-葡萄糖苷酶抑制活性。芒果苷和异芒果苷在须根中含量丰富，而分析的皂苷在根茎中含量丰富。基于主成分分析（PCA）、正交偏最小二乘判别分析（OPLS-DA）和偏最小二乘（PLS）等化学计量学方法，芒果苷和异芒果苷可能是该属植物α-葡萄糖苷酶抑制活性的主要成分。这些发现表明，所建立的HPLC-QQQ/MS方法被证明对知母药材的质量控制有用且有效，须根有潜力作为抗糖尿病药用资源加以利用。
