Minari André Luis Araujo, Oyama Lila Missae, Dos Santos Ronaldo Vagner Thomatieli
Departamento de Psicobiologia, Universidade Federal de São Paulo, UNIFESP, São Paulo, Brazil.
Departamento de Fisiologia, Disciplina de Fisiologia da Nutrição, Universidade Federal de São Paulo UNIFESP, São Paulo, Brazil.
J Muscle Res Cell Motil. 2017 Apr;38(2):231-239. doi: 10.1007/s10974-017-9475-7. Epub 2017 Jun 30.
After severe skeletal muscle damage, communication between inflammatory macrophages, myogenic cells, and modulatory secretion factors is essential to induce re-establishment of skeletal muscle structure. To analyze when characteristic gene expression of macrophages, myogenic cells, and SLPI are modulated after an exercise-induced muscle damage (EIMD) downhill protocol. Twenty-six rats were exposed to an experimental protocol of exercise and euthanized before (CTRL), immediately after (G0), and 24 (G24) and 48 (G48) hours after the exercise. After euthanasia, the Triceps brachii were dissected and analyzed by enzyme-linked immunosorbent assay and real time polymerase chain reaction. The CD68 expression was higher in the G24 when compared with all groups (p value < 0.05), whereas the CD163 was inhibited compared with G0 (p value < 0.05). MyoD and Myogenin were higher in the G24 when compared with G0 and G48 (p value < 0.05). The mRNA Secretory Leukocyte Protease Inhibitor (SLPI) was higher in the G48 when compared with the CTRL and G0 (p value < 0.05). IL-6 and TNF-α cytokines did not significantly change, but IL-10 presented a trend to be lower in the G0 when compared with G24 (p value = 0.054). A significant negative correlation was observed between CD68/CD163 (C.C = -0.71) and positive correlations between CD68/Myogenin (C.C = 0.65); MyoD/Myogenin (C.C = 0.72); IL-10/MyoD (C.C = 0.46), IL-10/MYOGENIN (C.C = 0.59); and IL-6/IL-10 (C.C = 0.64). A higher expression of CD68, concomitant with an elevation in MyoD and Myogenin 24 h after exercise, along with some correlations, suggests macrophage communication with myogenic cells independent of CD163 elevation. Additionally, the reestablishment of IL-10 in 24 h with the SLPI increased until 48 h indicate that these molecules are involved with anti-inflammatory transition after downhill exercise in the TBIH of Wistar rats.
严重骨骼肌损伤后，炎性巨噬细胞、肌原细胞和调节性分泌因子之间的通讯对于诱导骨骼肌结构的重新建立至关重要。为了分析在运动诱导的肌肉损伤（EIMD）下坡方案后，巨噬细胞、肌原细胞和分泌型白细胞蛋白酶抑制剂（SLPI）的特征性基因表达何时受到调节。26只大鼠接受运动实验方案，并在运动前（对照组）、运动后立即（G0）、运动后24小时（G24）和48小时（G48）实施安乐死。安乐死后，解剖肱三头肌，通过酶联免疫吸附测定和实时聚合酶链反应进行分析。与所有组相比，G24组中CD68表达更高（p值<0.05），而与G0组相比，CD163受到抑制（p值<0.05）。与G0组和G48组相比，G24组中MyoD和肌细胞生成素更高（p值<0.05）。与对照组和G0组相比，G48组中mRNA分泌型白细胞蛋白酶抑制剂（SLPI）更高（p值<0.05）。IL-6和TNF-α细胞因子没有显著变化，但与G24组相比，G0组中IL-10呈降低趋势（p值=0.054）。观察到CD68/CD163之间存在显著负相关（相关系数=-0.71），以及CD68/肌细胞生成素（相关系数=0.65）、MyoD/肌细胞生成素（相关系数=0.72）、IL-io/MyoD（相关系数=0.46）、IL-10/肌细胞生成素（相关系数=0.59）和IL-6/IL-10（相关系数=0.64）之间存在正相关。运动后24小时CD68的较高表达，同时伴有MyoD和肌细胞生成素的升高以及一些相关性，表明巨噬细胞与肌原细胞的通讯独立于CD163的升高。此外，24小时内IL-10的重新建立以及SLPI直至48小时的增加表明，这些分子参与了Wistar大鼠下坡运动后肱三头肌的抗炎转变。
