肠道微生物群组成改变与慢性荨麻疹的关联。
Association of altered gut microbiota composition with chronic urticaria.
作者信息
Nabizadeh Edris, Jazani Nima Hosseini, Bagheri Morteza, Shahabi Shahram
机构信息
Faculty of Medicine, Department of Microbiology, Urmia University of Medical Sciences, Urmia, Iran.
Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.
出版信息
Ann Allergy Asthma Immunol. 2017 Jul;119(1):48-53. doi: 10.1016/j.anai.2017.05.006.
BACKGROUND
An altered gut microbiota composition has recently been linked to some types of allergies.
OBJECTIVE
To compare the relative amounts of Akkermansia muciniphila, Clostridium leptum, Faecalibacterium prausnitzii, and Enterobacteriaceae as members of gut microbiota among patients with chronic urticaria (CU) and healthy controls.
METHODS
A total of 20 patients with CU and 20 healthy individuals matched by age and sex participated in the study. Fresh fecal samples were collected, and DNA extracted from stool samples was analyzed by real-time polymerase chain reaction for the qualitative and quantitative assays of the so-called bacteria.
RESULTS
The frequencies of A muciniphila, C leptum, and F prausnitzii in healthy controls' stool samples were significantly more than those of patients with CU (P < .001, P < .01, and P < .05, respectively), whereas the Enterobacteriaceae family was detected in all patients and healthy controls' stool samples. The relative amounts of A muciniphila in healthy control positive samples were significantly higher than those of samples from patients with CU (P < .001). Furthermore, there was a corresponding increase of relative amounts of C leptum and F prausnitzii in healthy control positive samples compared with those of patients with CU (P = .09 and P = .08, respectively). The mean of the relative amounts of Enterobacteriaceae family in the stool samples from patients with CU was more than that of healthy controls; however, the difference was nearly significant (P = .12).
CONCLUSION
The results reveal a change of frequency and relative amounts of A muciniphila, C leptum, and F prausnitzii in patients with CU compared with healthy controls. This is the first study, to our knowledge, to show the change of microbiota composition in patients with CU.
背景
肠道微生物群组成的改变最近被认为与某些类型的过敏有关。
目的
比较慢性荨麻疹（CU）患者和健康对照者肠道微生物群中嗜黏蛋白阿克曼氏菌、纤细梭菌、普拉梭菌和肠杆菌科的相对含量。
方法
共有20例CU患者和20例年龄及性别匹配的健康个体参与本研究。收集新鲜粪便样本，从粪便样本中提取DNA，通过实时聚合酶链反应对上述细菌进行定性和定量分析。
结果
健康对照者粪便样本中嗜黏蛋白阿克曼氏菌、纤细梭菌和普拉梭菌的检出频率显著高于CU患者（分别为P < 0.001、P < 0.01和P < 0.05），而所有患者和健康对照者的粪便样本中均检测到肠杆菌科。健康对照阳性样本中嗜黏蛋白阿克曼氏菌的相对含量显著高于CU患者样本（P < 0.001）。此外，与CU患者相比，健康对照阳性样本中纤细梭菌和普拉梭菌的相对含量相应增加（分别为P = 0.09和P = 0.08）。CU患者粪便样本中肠杆菌科的相对含量均值高于健康对照者；然而，差异接近显著（P = 0.12）。
结论
结果显示，与健康对照者相比，CU患者中嗜黏蛋白阿克曼氏菌、纤细梭菌和普拉梭菌的频率和相对含量发生了变化。据我们所知，这是第一项显示CU患者微生物群组成变化的研究。
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