Sano T, Saito H, Yamasaki R, Hamaguchi K, Ooiwa K, Shimoda T, Hosoi E, Saito S, Hizawa K
Cancer. 1986 Jan 1;57(1):64-8. doi: 10.1002/1097-0142(19860101)57:1<64::aid-cncr2820570114>3.0.co;2-6.
A well-differentiated neuroendocrine carcinoma of the lung that secreted immunoreactive somatostatin (IR-SRIF) and IR-calcitonin (CT) in a 72-year-old women is described. The plasma concentrations of IR-SRIF (57.5 pg/ml) and IR-CT (340 pg/ml) before operation were significantly higher than the respective normal ranges. After resection of the tumor, the plasma CT level (105 pg/ml) decreased to within the normal range, and the SRIF level (32.7 pg/ml) also decreased, but was still abnormally higher, which suggested the presence of an unidentified remnant of the tumor. Abnormal accumulation of technetium 99m (99mTc) in the lumbar vertebrae was found 6 months after the operation, which indicated a metastatic tumor. The tissue concentrations of IR-SRIF and IR-CT were 103 and 94 ng/g wet weight, respectively, and SRIF-IR tumor cells and CT-IR tumor cells were demonstrated immunohistochemically. On gel-filtration chromatography of the tumor tissue, two peaks of SRIF immunoreactivity were eluted in the positions of synthetic SRIF-28 and SRIF-14, respectively. Conversion of SRIF-28 to SRIF-14 was suggested from results on changes in the two IR-SRIF components during incubation with a crude enzyme preparation extracted from the tumor tissue.
本文描述了一名72岁女性患者，其患有分泌免疫反应性生长抑素（IR-SRIF）和免疫反应性降钙素（CT）的高分化肺神经内分泌癌。术前IR-SRIF（57.5 pg/ml）和IR-CT（340 pg/ml）的血浆浓度显著高于各自的正常范围。肿瘤切除后，血浆CT水平（105 pg/ml）降至正常范围内，SRIF水平（32.7 pg/ml）也有所下降，但仍异常偏高，这提示存在未明确的肿瘤残余。术后6个月发现腰椎有99m锝（99mTc）异常聚集，提示有转移瘤。IR-SRIF和IR-CT的组织浓度分别为103和94 ng/g湿重，免疫组化显示有SRIF-IR肿瘤细胞和CT-IR肿瘤细胞。对肿瘤组织进行凝胶过滤层析时，在合成的SRIF-28和SRIF-14位置分别洗脱出色谱图上出现两个SRIF免疫反应峰。从肿瘤组织中提取的粗酶制剂孵育期间两种IR-SRIF成分的变化结果提示SRIF-28向SRIF-14的转化。
