Universidade Federal de Viçosa, Departamento de Veterinária, Campus UFV, 36570-900, Viçosa, MG, Brazil.
Universidade Federal de Viçosa, Departamento de Bioquímica e Biologia Molecular, Campus UFV, 36570-900, Viçosa, MG, Brazil.
Int J Antimicrob Agents. 2018 Jan;51(1):33-37. doi: 10.1016/j.ijantimicag.2017.04.020. Epub 2017 Jun 28.
Bacteriocins have unusual inhibitory activity, including antiviral properties, and this can be exploited to give alternative applications. Semi-purified bacteriocins of six lactic acid bacteria (LAB) strains isolated from goat milk (two Lactococcus lactis: GLc03 and GLc05, and four Enterococcus durans: GEn09, GEn12, GEn14 and GEn17) were tested for cytotoxicity in Vero cells (50% Cytotoxicity Concentration: CC), and for their antiviral activities against herpes simplex virus 1 (HVS-1) and poliovirus (PV-1). Semi-purified bacteriocins presented low cytotoxicity, with CC varying from 256.2 µg/mL (GLc05) to 1084.5 µg/mL (GEn14). CC was determined for all isolates (GLc03: 36.9 µg/mL; GLc05: 51.2 µg/mL; GEn09: 88.1 µg/mL; GEn12: 99.9 µg/mL; GEn14: 275 µg/mL; and GEn17: 62.2 µg/mL) and considered for antiviral activity assays. Antiviral activity before virus adsorption was recorded against PV-1 for GLc05 (4.9%), GEn09 (3.4%), GEn12 (24.7%) and GEn17 (23.5%), and against HSV-1 for GEn12 (27.9%), GEn14 (58.7%) and GEn17 (39.2%). Antiviral activity after virus adsorption was identified against PV-1 for GLc05 (32.7%), GEn09 (91.0%), GEn12 (93.7%) and GEn17 (57.2%), and against HSV-1 for GEn17 (71.6%). The results obtained indicate the potential of some bacteriocins, particularly those produced by E. durans strains investigated in the present study, in viral inhibition and their application as new antiviral agents.
细菌素具有独特的抑制活性，包括抗病毒特性，这可以被利用来提供替代的应用。从羊奶中分离出的 6 株乳酸细菌（LAB）的半纯化细菌素（2 株乳球菌：GLc03 和 GLc05，4 株肠球菌：GEn09、GEn12、GEn14 和 GEn17）在 Vero 细胞中进行细胞毒性测试（半最大细胞毒性浓度：CC），并测试其对单纯疱疹病毒 1（HSV-1）和脊髓灰质炎病毒 1（PV-1）的抗病毒活性。半纯化的细菌素表现出低细胞毒性，CC 值从 256.2μg/mL（GLc05）到 1084.5μg/mL（GEn14）不等。对所有分离株（GLc03：36.9μg/mL；GLc05：51.2μg/mL；GEn09：88.1μg/mL；GEn12：99.9μg/mL；GEn14：275μg/mL；GEn17：62.2μg/mL）进行了 CC 测定，并考虑用于抗病毒活性测定。在病毒吸附前记录针对 PV-1 的 GLc05（4.9%）、GEn09（3.4%）、GEn12（24.7%）和 GEn17（23.5%）以及针对 HSV-1 的 GEn12（27.9%）、GEn14（58.7%）和 GEn17（39.2%）的抗病毒活性。在病毒吸附后，针对 GLc05（32.7%）、GEn09（91.0%）、GEn12（93.7%）和 GEn17（57.2%）的 PV-1 以及针对 GEn17（71.6%）的 HSV-1 检测到抗病毒活性。研究结果表明，一些细菌素，特别是本研究中分离的肠球菌菌株产生的细菌素，具有抑制病毒的潜力，可以作为新型抗病毒药物应用。
