Wachsman M B, Farías M E, Takeda E, Sesma F, de Ruiz Holgado A P, de Torres R A, Coto C E
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Argentina.
Int J Antimicrob Agents. 1999 Aug;12(4):293-9. doi: 10.1016/s0924-8579(99)00078-3.
Enterocin CRL35 is an antibacterial polypeptide of 3.5 x 10(3) Da produced by Enterococcus faecium CRL35. A series of experiments are described that show the enterocin also had antiviral activity against thymidine-kinase positive (tk+) and deficient (tk-) strains of herpes simplex (HSV) type 1 and 2 in Vero and BHK-21 cells. This activity was observed at 100 microg/ml, 15-fold lower than the cytotoxic concentration. In both cell lines there was a 2 log inhibition of infectivity. The compound inhibited viral multiplication in a dose-dependent manner and had no virucidal effect. Enterocin CRL35 also inhibited the virion-associated host shutoff in infected Vero cells showing that intracellular viral multiplication was affected.
肠球菌素CRL35是由屎肠球菌CRL35产生的一种分子量为3.5×10(3)Da的抗菌多肽。本文描述了一系列实验，结果表明该肠球菌素对1型和2型单纯疱疹病毒（HSV）的胸苷激酶阳性（tk+）和缺陷型（tk-）毒株在Vero细胞和BHK-21细胞中也具有抗病毒活性。在100μg/ml时可观察到这种活性，该浓度比细胞毒性浓度低15倍。在两种细胞系中，感染性均有2个对数级的抑制。该化合物以剂量依赖的方式抑制病毒增殖，且无杀病毒作用。肠球菌素CRL35还抑制了感染的Vero细胞中与病毒粒子相关的宿主关闭，表明细胞内病毒增殖受到影响。
