用酵母益生菌混合菌株补充雀巢婴儿米粉，在体外以及母婴白化大鼠体内均诱导出高黄曲霉毒素解毒潜力。

Supplementation of Cerelac baby food with yeast-probiotic cocktail strains induces high potential for aflatoxin detoxification both in vitro and in vivo in mother and baby albino rats.

作者信息

Hamad Gamal M, Taha Tarek H, Hafez Elsayed E, Ali Safwat H, El Sohaimy Sobhy A

机构信息

Food Technology Department, Arid Land Cultivation Research Institute (ALCRI), City of Scientific Research and Technology Applications (SRTA- CITY), New Borg El-Arab City, Alexandria, Egypt.

Environmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technology Applications (SRTA- CITY), New Borg El-Arab City, Alexandria, Egypt.

出版信息

J Sci Food Agric. 2018 Jan;98(2):707-718. doi: 10.1002/jsfa.8518. Epub 2017 Aug 22.

DOI:10.1002/jsfa.8518

PMID:28671306

Abstract

BACKGROUND

Aflatoxins (AFs) are a group of toxic, nephrotoxic, hepatotoxic and carcinogenic fungal metabolites. Heat- and acid-treated yeasts, probiotic bacteria and their combination were used to remove AFB1, AFB2, AFG1 and AFG2 from human and animal food.

RESULTS

The in vitro study revealed that the highest removal percentage of AFs in phosphate-buffered saline was recorded after 72 h with the yeast-probiotic coctile, reaching 95.59%. Therefore, this coctile was added to Cerelac contaminated with AFB1, AFB2, AFG1 and AFG2, and the removal percentages were 8.17%, 36.12%, 44.75%, 64.72% and 93.21% after 6, 12, 24, 48 and 72 h of treatment, respectively. Cerelac yeast-probiotic coctile was administered to female rats and the results showed that all AFs (AFB1, AFB2, AFG1 and AFG2) were detected in the serum of mother rats for both AF groups III and IV. On the other hand, AFM1 and AFM2 metabolites were not observed in mothers' sera but were detected in all infants of groups III and IV. Meanwhile, AFB1, AFB2, AFG1 and AFG2 were not observed in infants' sera.

CONCLUSION

摘要

背景

黄曲霉毒素（AFs）是一组具有毒性、肾毒性、肝毒性和致癌性的真菌代谢产物。经热处理和酸处理的酵母、益生菌及其组合被用于去除人类和动物食品中的黄曲霉毒素B1（AFB1）、黄曲霉毒素B2（AFB2）、黄曲霉毒素G1（AFG1）和黄曲霉毒素G2（AFG2）。

结果

体外研究表明，酵母-益生菌混合物在磷酸盐缓冲盐水中处理72小时后，对黄曲霉毒素的去除率最高，达到95.59%。因此，将该混合物添加到被AFB1、AFB2、AFG1和AFG2污染的婴儿米粉中，处理6、12、24、48和72小时后的去除率分别为8.17%、36.12%、44.75%、64.72%和93.21%。给雌性大鼠喂食婴儿米粉-酵母-益生菌混合物，结果显示，AF组III和IV的母鼠血清中均检测到所有的黄曲霉毒素（AFB1、AFB2、AFG1和AFG2）。另一方面，母鼠血清中未观察到AFM1和AFM2代谢产物，但在III组和IV组的所有婴儿中均检测到。同时，婴儿血清中未观察到AFB1、AFB2、AFG1和AFG2。