Key laboratory of Neuroregeneration of Jiangsu and Ministry of Education, Nantong University, 226001, Nantong, PR China; The Neural Regeneration Co-innovation Center of Jiangsu Province, 226001 Nantong, PR China; Department of Mechanical Engineering, Boston University, Boston, MA 02215, USA.
Department of Pediatrics, Affiliated Hospital of Nantong University, 226001, Nantong, PR China.
Colloids Surf B Biointerfaces. 2017 Oct 1;158:57-67. doi: 10.1016/j.colsurfb.2017.06.026. Epub 2017 Jun 22.
To further improve the property of promoting peripheral nerve regeneration of chitosan materials, CaTiO nanoparticles with various concentrations were synthesized in chitosan (CS) solution and formed to porous CS/CaTiO hybrid scaffolds. The properties including morphology, wettability, porosity, crystallization intensity and surface charges were characterized, respectively. The influence of the porous CS/CaTiO hybrid scaffolds on Schwann cells growth was evaluated. The results showed that the CaTiO hybridized CS scaffolds possessed homogeneous nanoparticles distribution with concentration-dependent effect. The hybridization of CaTiO nanoparticles could increase the hydrophobicity while reduce the porosity and surface charge density of the porous CS/CaTiO hybrid scaffolds The crystal structure of the hybridized scaffolds was mainly the orthorhombic structure of the calcium titanate accompanied by the amorphous phase of chitosan. Culture of Schwann cells indicated that the CS/CaTiO hybrid scaffolds with a suitable concentration of CaTiO nanoparticles could obviously promote the attachment, proliferation and biological function maintenance of Schwann cells, thus showing potentially great significance towards application in peripheral nerve regeneration.
为了进一步提高壳聚糖材料促进周围神经再生的性能，在壳聚糖（CS）溶液中合成了具有不同浓度的 CaTiO 纳米粒子，并将其形成多孔 CS/CaTiO 杂化支架。分别对其形貌、润湿性、孔隙率、结晶强度和表面电荷进行了表征。评估了多孔 CS/CaTiO 杂化支架对许旺细胞生长的影响。结果表明，CS/CaTiO 杂化支架具有均匀的纳米粒子分布，具有浓度依赖性。CaTiO 纳米粒子的杂交可以增加疏水性，同时降低多孔 CS/CaTiO 杂化支架的孔隙率和表面电荷密度。杂交支架的晶体结构主要是钙钛矿的正交结构，伴有壳聚糖的非晶相。许旺细胞培养表明，具有合适浓度 CaTiO 纳米粒子的 CS/CaTiO 杂化支架可以明显促进许旺细胞的附着、增殖和生物功能维持，因此在周围神经再生应用方面具有潜在的重要意义。
